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ProductsBack/KAP1 antibody [N3C2], Internal

KAP1 antibody [N3C2], Internal

GTX102226
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetTRIM28
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Overview

  • Supplier
    GeneTex
  • Product Name
    KAP1 antibody [N3C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.48 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10155
  • Target name
    TRIM28
  • Target description
    tripartite motif containing 28
  • Target synonyms
    E3 SUMO-protein ligase TRIM28; KAP1; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-interacting protein 1; KRIP-1; nuclear corepressor KAP-1; PPP1R157; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; RNF96; TF1B; TIF1B; TIF1-beta; transcription intermediary factor 1-beta; transcriptional intermediary factor 1-beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13263
  • Protein Name
    Transcription intermediary factor 1-beta
  • Scientific Description
    The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • DNA-PK promotes DNA end resection at DNA double strand breaks in G(0) cells. Fowler FC et al., 2022 May 16, Elife
    Read more
  • The RNF8 and RNF168 Ubiquitin Ligases Regulate Pro- and Anti-Resection Activities at Broken DNA Ends During Non-Homologous End Joining. Chen BR et al., 2021 Dec, DNA Repair (Amst)
    Read more
  • LIN37-DREAM prevents DNA end resection and homologous recombination at DNA double-strand breaks in quiescent cells. Chen BR et al., 2021 Sep 3, Elife
    Read more
  • Expression and Significance of TRIM 28 in Squamous Carcinoma of Esophagus. Liu B et al., 2019 Oct, Pathol Oncol Res
    Read more
  • Ginsenoside Rh2 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Regulating the TLR4/PI3K/Akt/mTOR, Raf-1/MEK/ERK, and Keap1/Nrf2/HO-1 Signaling Pathways in Mice. Hsieh YH et al., 2018 Sep 1, Nutrients
    Read more
  • MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining. Hung PJ et al., 2018 Jul 19, Mol Cell
    Read more
  • The histone chaperone ASF1 regulates the activation of ATM and DNA-PKcs in response to DNA double-strand breaks. Huang TH et al., 2018, Cell Cycle
    Read more
  • BET Protein BRDT Complexes With HDAC1, PRMT5, and TRIM28 and Functions in Transcriptional Repression During Spermatogenesis. Wang L et al., 2016 Jun, J Cell Biochem
    Read more
  • KAP-1 promotes resection of broken DNA ends not protected by gamma-H2AX and 53BP1 in G1-phase lymphocytes. Tubbs AT et al., 2014 Aug, Mol Cell Biol
    Read more
  • Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells. Aslanian A et al., 2014 Mar, DNA Repair (Amst)
    Read more

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Figure 1. Western blot analysis of KAP1 using anti-KAP1 antibody (PB9835). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAP1 antigen affinity purified polyclonal antibody (Catalog # PB9835) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAP1 at approximately 100 kDa. The expected band size for KAP1 is at 89 kDa.
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