Immunohistochemical analysis of paraffin-embedded human breast cancer, using KAP1(GTX102226) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KAP1 stained by KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX102226/GTX102226_44490_20211210_IHC-P_M_1_w_23060100_290.webp "KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KAP1 stained by KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. KAP1 stained by KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX102226/GTX102226_44490_20211210_IHC-P_M_w_23060100_818.webp "KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. KAP1 stained by KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102226/GTX102226_44489_20211112_WB_w_23060100_614.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KAP1 stained by KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:2000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102226/GTX102226_43628_20200219_ICC_IF_w_23060100_583.webp "KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KAP1 stained by KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:2000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 μm.")
Immunohistochemical analysis of paraffin-embedded human breast cancer, using KAP1(GTX102226) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
KAP1 antibody [N3C2], Internal
GTX102226
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetTRIM28
Overview
- SupplierGeneTex
- Product NameKAP1 antibody [N3C2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.48 mg/ml
- ConjugateUnconjugated
- Gene ID10155
- Target nameTRIM28
- Target descriptiontripartite motif containing 28
- Target synonymsKAP1, PPP1R157, RNF96, TF1B, TIF1B, TIF1beta, WT7, transcription intermediary factor 1-beta, E3 SUMO-protein ligase TRIM28, KAP-1, KRAB [Kruppel-associated box domain]-associated protein 1, KRAB-interacting protein 1, KRIP-1, RING finger protein 96, RING-type E3 ubiquitin transferase TIF1-beta, TIF1-beta, nuclear corepressor KAP-1, protein phosphatase 1, regulatory subunit 157, transcriptional intermediary factor 1-beta
- HostRabbit
- IsotypeIgG
- Protein IDQ13263
- Protein NameTranscription intermediary factor 1-beta
- Scientific DescriptionThe protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Fowler FC, Chen BR, Zolnerowich N, et al. DNA-PK promotes DNA end resection at DNA double strand breaks in G(0) cells. Elife. 2022,11. doi: 10.7554/eLife.74700Read this paper
- Chen BR, Wang Y, Shen ZJ, et al. The RNF8 and RNF168 Ubiquitin Ligases Regulate Pro- and Anti-Resection Activities at Broken DNA Ends During Non-Homologous End Joining. DNA Repair (Amst). 2021,108:103217. doi: 10.1016/j.dnarep.2021.103217Read this paper
- Chen BR, Wang Y, Tubbs A, et al. LIN37-DREAM prevents DNA end resection and homologous recombination at DNA double-strand breaks in quiescent cells. Elife. 2021,10. doi: 10.7554/eLife.68466Read this paper
- Liu B, Li X, Liu F, et al. Expression and Significance of TRIM 28 in Squamous Carcinoma of Esophagus. Pathol Oncol Res. 2019,25(4):1645-1652. doi: 10.1007/s12253-018-0558-6Read this paper
- Hsieh YH, Deng JS, Chang YS, et al. Ginsenoside Rh2 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Regulating the TLR4/PI3K/Akt/mTOR, Raf-1/MEK/ERK, and Keap1/Nrf2/HO-1 Signaling Pathways in Mice. Nutrients. 2018,10(9). doi: 10.3390/nu10091208Read this paper
- Hung PJ, Johnson B, Chen BR, et al. MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining. Mol Cell. 2018,71(2):332-342.e8. doi: 10.1016/j.molcel.2018.06.018Read this paper
- Huang TH, Shen ZJ, Sleckman BP, et al. The histone chaperone ASF1 regulates the activation of ATM and DNA-PKcs in response to DNA double-strand breaks. Cell Cycle. 2018,17(12):1413-1424. doi: 10.1080/15384101.2018.1486165Read this paper
- Wang L, Wolgemuth DJ. BET Protein BRDT Complexes With HDAC1, PRMT5, and TRIM28 and Functions in Transcriptional Repression During Spermatogenesis. J Cell Biochem. 2016,117(6):1429-38. doi: 10.1002/jcb.25433Read this paper
- Tubbs AT, Dorsett Y, Chan E, et al. KAP-1 promotes resection of broken DNA ends not protected by γ-H2AX and 53BP1 in G₁-phase lymphocytes. Mol Cell Biol. 2014,34(15):2811-21. doi: 10.1128/MCB.00441-14Read this paper
- Aslanian A, Yates JR 3rd, Hunter T. Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells. DNA Repair (Amst). 2014,15:29-38. doi: 10.1016/j.dnarep.2013.12.007Read this paper
Datasheet
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