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IL-2 Antibody

ORB1671480
Biorbyt
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetIL2
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Overview

  • Supplier
    Biorbyt
  • Product Name
    IL-2 Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Sandwich ELISA: In a sandwich ELISA (assuming 100microl/well), a concentration of 4.0-8.0 microg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-2 when used with Biorbyts Biotinylated Antigen Affinity Purified Anti-Human IL-2 as the detection antibody at a concentration of approximately 1.0- 2.0 microg/ml. Western Blot To detect Human IL-2 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 microg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID3558
  • Target name
    IL2
  • Target description
    interleukin 2
  • Target synonyms
    IL-2, TCGF, lymphokine, interleukin-2, T cell growth factor, aldesleukin, involved in regulation of T-cell clonal expansion
  • Host
    Mouse
  • Protein IDP60568
  • Protein Name
    Interleukin-2
  • Scientific Description
    IL-2 Antibody
  • Reactivity
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL-2 using anti-IL-2 antibody (A00387-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole Cell lysates, Lane 2: K562 whole Cell lysates, Lane 3: RAJI whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-2 antigen affinity purified polyclonal antibody (Catalog # A00387-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-2 at approximately 22KD. The expected band size for IL-2 is at 18KD.
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