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Anti-IL-2 [LNKB-2]

Ab00232-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, Other Application
Product group Antibodies
TargetIL2
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-IL-2 [LNKB-2]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody was quantified by ELISA and used for latex-agglutination analysis of human IL-2. It was measured to bind human IL-2 with high affinity (Ka ~ 3 x 108 M-1) (Lunev et al., 1990; PMID: 1718463). This antibody has been used successfully for WB and FACS (Stratieva-Taneeva et al., 1993; PMID: 8359822). The crystal structure characterization of the Fab version of this antibody was performed (Fokin et al., 2000; PMID: 11040993). Further crystallization of the Fab version of this antibody with an antigenic peptide revealed that the antibody-antigen complexation involves a significant rearrangement of the epitope-containing region of the IL-2 with retention of the alpha-helical character of the epitope fragment (Afonin et al., 2001; PMID: 11468348).
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, Other Application
  • Applications Supplier
    WB; IP; IF; ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    LNKB-2
  • Gene ID3558
  • Target name
    IL2
  • Target description
    interleukin 2
  • Target synonyms
    aldesleukin; IL-2; interleukin-2; involved in regulation of T-cell clonal expansion; lymphokine; T cell growth factor; TCGF
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP60568
  • Protein Name
    Interleukin-2
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL-2 using anti-IL-2 antibody (A00387-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole Cell lysates, Lane 2: K562 whole Cell lysates, Lane 3: RAJI whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-2 antigen affinity purified polyclonal antibody (Catalog # A00387-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-2 at approximately 22KD. The expected band size for IL-2 is at 18KD.
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