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Anti-human IL-2 mAb (MT2A91) for WB, unconjugated

Anti-human IL-2 mAb (MT2A91) for WB, unconjugated

3445-1
Mabtech
ApplicationsWestern Blot
Product group Antibodies
TargetIL2
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-human IL-2 mAb (MT2A91) for WB, unconjugated
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MT2A91
  • Conjugate
    Unconjugated
  • Gene ID3558
  • Target name
    IL2
  • Target description
    interleukin 2
  • Target synonyms
    IL-2, TCGF, lymphokine, interleukin-2, T cell growth factor, aldesleukin, involved in regulation of T-cell clonal expansion
  • Host
    Mouse
  • Isotype
    IgG2b
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables specific detection of human IL-2 using Western blot.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL-2 using anti-IL-2 antibody (A00387-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole Cell lysates, Lane 2: K562 whole Cell lysates, Lane 3: RAJI whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-2 antigen affinity purified polyclonal antibody (Catalog # A00387-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-2 at approximately 22KD. The expected band size for IL-2 is at 18KD.
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