More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/IL-2 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with _x000D_ IL-2 Polyclonal Antibody, Unconjugated (bs-10274R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with _x000D_ IL-2 Polyclonal Antibody, Unconjugated (bs-10274R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with _x000D_ IL-2 Polyclonal Antibody, Unconjugated (bs-10274R) at 1:200 overnight at 4°C, DAB staining.

IL-2 Polyclonal Antibody

BS-10274R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIL2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    IL-2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3558
  • Target name
    IL2
  • Target description
    interleukin 2
  • Target synonyms
    IL-2, TCGF, lymphokine, interleukin-2, T cell growth factor, aldesleukin, involved in regulation of T-cell clonal expansion
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60568
  • Protein Name
    Interleukin-2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of HT29 cells using IL-2 Polyclonal Antibody
Product group Antibodies
Cusabio
IL2 AntibodyCSB-PA003024
ApplicationsWestern Blot, ELISA
ReactivityHuman, Rat
TargetIL2
  • SizePrice
Product group Antibodies
GeneTex
IL2 antibodyGTX101138
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetIL2
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-IL-2 [LNKB-2]Ab00232-1.1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, Other Application
TargetIL2
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-IL-2 Recombinant Monoclonal Antibody [BLR287L]A700-287
ApplicationsImmunoPrecipitation, Western Blot
TargetIL2
  • SizePrice
Anti-IL-2 Antibody
Product group Antibodies
Antibodies.com
Anti-IL-2 AntibodyA99883
ApplicationsWestern Blot, ELISA
  • SizePrice
Figure 1. Western blot analysis of IL-2 using anti-IL-2 antibody (A00387-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole Cell lysates, Lane 2: K562 whole Cell lysates, Lane 3: RAJI whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-2 antigen affinity purified polyclonal antibody (Catalog # A00387-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-2 at approximately 22KD. The expected band size for IL-2 is at 18KD.
Product group Antibodies
Boster Bio
Anti-IL-2/IL2 Antibody Picoband(r)A00387-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetIL2
  • SizePrice
Product group Antibodies
RayBiotech
Anti-IL-2 Antibody130-11056
ApplicationsELISA
  • SizePrice
Product group Antibodies
Biosynth
IL2 antibody10-I72A
  • SizePrice
Product group Antibodies
Biomatik
Interleukin 2 (IL2) Polyclonal AntibodyCAU28727
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetIL2
  • SizePrice
Product group Antibodies
Assay Genie
IL2 AntibodyCAB0302
TargetIL2
  • SizePrice