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ProductsBack/Il2rb Rat Monoclonal Antibody [Clone ID: 5H4]
Immunofluorescent Staining: BALB/c lymph node cells were double stained with Rat anti-Mouse CD3e-PE and Rat anti-Mouse CD122-FITC. Small lymphocytes were gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 ug/10e6 cells.
Immunofluorescent Staining: BALB/c lymph node cells were double stained with Rat anti-Mouse CD3e-PE and Rat anti-Mouse CD122-FITC. Small lymphocytes were gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 ug/10e6 cells.
Immunofluorescent Staining: BALB/c lymph node cells were double stained with Rat anti-Mouse CD3e-PE and Rat anti-Mouse CD122-FITC. Small lymphocytes were gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 ug/10e6 cells.

Il2rb Rat Monoclonal Antibody [Clone ID: 5H4]

AM08078FC-N
OriGene
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityMouse
TargetIl2rb
500 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Il2rb Rat Monoclonal Antibody [Clone ID: 5H4]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5H4
  • Gene ID16185
  • Target name
    Il2rb
  • Target description
    interleukin 2 receptor, beta chain
  • Target synonyms
    CD122, IL-15Rbeta, IL15Rbeta, Il-2/15Rbeta, Il-2Rbeta, p70, interleukin-2 receptor subunit beta, IL-15 receptor beta chain, IL-2 receptor beta chain, IL-2 receptor subunit beta, IL-2/15 receptor-beta, IL-2R subunit beta, IL-2RB, high affinity IL-2 receptor subunit beta, p70-75
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDP16297
  • Protein Name
    Interleukin-2 receptor subunit beta
  • Scientific Description
    Il2rb rat monoclonal antibody, clone 5H4, FITC
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of IL2RB using anti-IL2RB antibody (PB9761). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: Mouse Lung Tissue Lysate, Lane 2: Mouse Spleen Tissue Lysate, Lane 3: Mouse Testis Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2RB antigen affinity purified polyclonal antibody (Catalog # PB9761) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL2RB at approximately 75 kDa. The expected band size for IL2RB is at 75 kDa.
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