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INTU Antibody, HRP conjugated

CSB-PA891544LB01HU
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetINTU
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Overview

  • Supplier
    Cusabio
  • Product Name
    INTU Antibody, HRP conjugated
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Gene ID27152
  • Target name
    INTU
  • Target description
    inturned planar cell polarity protein
  • Target synonyms
    CPLANE4, INT, OFD17, PDZD6, PDZK6, SRTD20, protein inturned, PDZ domain containing 6, PDZ domain-containing protein 6, ciliogenesis and planar polarity effector 4, ciliogenesis and planar polarity effector complex subunit 4, inturned planar cell polarity effector homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9ULD6
  • Protein Name
    Protein inturned
  • Scientific Description
    Plays a key role in ciliogenesis and embryonic development. Regulator of cilia formation by controlling the organization of the apical actin cytoskeleton and the positioning of the basal bodies at the apical cell surface, which in turn is essential for the normal orientation of elongating ciliary microtubules. Plays a key role in definition of cell polarity via its role in ciliogenesis but not via conversion extension. Has an indirect effect on hedgehog signaling (By similarity). Proposed to function as core component of the CPLANE (ciliogenesis and planar polarity effectors) complex involved in the recruitment of peripheral IFT-A proteins to basal bodies (PubMed:27158779).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of INTU using anti-INTU antibody (A11533-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INTU antigen affinity purified polyclonal antibody (Catalog # A11533-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INTU at approximately 100 kDa. The expected band size for INTU is at 80,106 kDa.
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ReactivityHuman
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