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WB analysis of 293T lysate (60ug) using ITLN1 antibody at the indicated dilutions.
WB analysis of 293T lysate (60ug) using ITLN1 antibody at the indicated dilutions.
WB analysis of 293T lysate (60ug) using ITLN1 antibody at the indicated dilutions.

ITLN1 antibody [AT6C11]

GTX53733
GeneTex
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetITLN1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ITLN1 antibody [AT6C11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:250.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT6C11
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID55600
  • Target name
    ITLN1
  • Target description
    intelectin 1
  • Target synonyms
    endothelial lectin HL-1; galactofuranose-binding lectin; hIntL; HL1; HL-1; intelectin 1 (galactofuranose binding); intelectin-1; intestinal lactoferrin receptor; INTL; ITLN; ITLN-1; LFR; omentin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ8WWA0
  • Protein Name
    Intelectin-1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ITLN1 using anti-ITLN1 antibody (PB10004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITLN1 antigen affinity purified polyclonal antibody (Catalog # PB10004) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITLN1 at approximately 43 kDa. The expected band size for ITLN1 is at 35 kDa.
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