More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Junctin / ASPH Antibody (N-Terminus)

Junctin / ASPH Antibody (N-Terminus)

LS-C81640
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetASPH
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Junctin / ASPH Antibody (N-Terminus)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Applications
    Western Blot
  • Applications Supplier
    WB ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID444
  • Target name
    ASPH
  • Target description
    aspartate beta-hydroxylase
  • Target synonyms
    A beta H-J-J; AAH; ASP beta-hydroxylase; aspartyl/asparaginyl beta-hydroxylase; BAH; cardiac junctin; CASQ2BP1; FDLAB; HAAH; humbug; JCTN; junctate; junctin; peptide-aspartate beta-dioxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

IHC-P analysis of human kidney tissue using GTX45856 ASPH antibody at 4.0-8.0microg/ml.
Product group Antibodies
GeneTex
ASPH antibody, N-termGTX45856
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetASPH
  • SizePrice
Product group Antibodies
Biomatik
Asph Polyclonal AntibodyCAC07339
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetASPH
  • SizePrice
Product group Antibodies
Assay Genie
ASPH AntibodyCAB13153
TargetASPH
  • SizePrice
Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue All lanes: ASPH antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86, 35, 26, 24, 34, 84, 30, 22, 29, 33 kDa Observed band size: 86 kDa
Product group Antibodies
Cusabio
ASPH AntibodyCSB-PA002226HA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetASPH
  • SizePrice
Product group Antibodies
RayBiotech
Anti-ASPH (Center) Antibody102-21227
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetASPH
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-ASPH Antibody, Affinity PurifiedA305-342A
ApplicationsImmunoPrecipitation, Western Blot
TargetASPH
  • SizePrice
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in astrocytes.
Product group Antibodies
Atlas Antibodies
Anti-ASPH AntibodyHPA059303
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetASPH
  • SizePrice
Figure 1. Western blot analysis of ASPH using anti-ASPH antibody (PB9478). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Liver Tissue Lysate, Lane 3: HELA Whole Cell Lysate, Lane 4: HEPG2 Whole Cell Lysate, Lane 5: HEPA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASPH antigen affinity purified polyclonal antibody (Catalog # PB9478) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASPH at approximately 100KD. The expected band size for ASPH is at 86KD.
Product group Antibodies
Boster Bio
Anti-Aspartate beta hydroxylase/ASPH Antibody Picoband(r)PB9478-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetASPH
  • SizePrice
Product group Antibodies
Antibodies.com
Anti-ASPH AntibodyA31720
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-Aspartate beta-hydroxylase [G3-F11]AB03472-1.1-BT
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetASPH
  • SizePrice