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WB analysis of HepG2 cell lysates using GTX87069 KCNN4 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of HepG2 cell lysates using GTX87069 KCNN4 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of HepG2 cell lysates using GTX87069 KCNN4 antibody. The lane on the right is blocked with the synthesized peptide.

KCNN4 antibody

GTX87069
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetKCNN4
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Overview

  • Supplier
    GeneTex
  • Product Name
    KCNN4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3783
  • Target name
    KCNN4
  • Target description
    potassium calcium-activated channel subfamily N member 4
  • Target synonyms
    DHS2, IK, IK1, IKCA1, KCA4, KCa3.1, SK4, SKCa4, hIK1, hIKCa1, hKCa4, hSK4, intermediate conductance calcium-activated potassium channel protein 4, gardos channel, intermediate-conductance Ca2+-activated K+ channel, KCa3.1, potassium channel, calcium activated intermediate/small conductance subfamily N alpha, member 4, potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4, putative Gardos channel, putative erythrocyte intermediate conductance calcium-activated potassium Gardos channel, small conductance calcium-activated potassium channel 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15554
  • Protein Name
    Intermediate conductance calcium-activated potassium channel protein 4
  • Scientific Description
    The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KCNN4 using anti-KCNN4 antibody (A01936-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysate, Lane 2: human PC-3 whole cell lysate, Lane 3: human A549 whole cell lysate, Lane 4: human Hela whole cell lysate, Lane 5: rat stomach tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNN4 antigen affinity purified polyclonal antibody (Catalog # A01936-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNN4 at approximately 48KD. The expected band size for KCNN4 is at 48KD.
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