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FACS analysis of Raji cells using GTX00627 KCNN4 antibody [6C1]. Black : Negative control Gray : Isotype control Green : Primary antibody Dilution : 1:20
FACS analysis of Raji cells using GTX00627 KCNN4 antibody [6C1]. Black : Negative control Gray : Isotype control Green : Primary antibody Dilution : 1:20
FACS analysis of Raji cells using GTX00627 KCNN4 antibody [6C1]. Black : Negative control Gray : Isotype control Green : Primary antibody Dilution : 1:20

KCNN4 antibody [6C1]

GTX00627
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application
Product group Antibodies
TargetKCNN4
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Overview

  • Supplier
    GeneTex
  • Product Name
    KCNN4 antibody [6C1] - Overexpression Validated
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6C1
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3783
  • Target name
    KCNN4
  • Target description
    potassium calcium-activated channel subfamily N member 4
  • Target synonyms
    DHS2; hIKCa1; hKCa4; hSK4; IK; IK1; IKCA1; intermediate conductance calcium-activated potassium channel protein 4; intermediate-conductance Ca2+-activated K+ channel, KCa3.1; KCa3.1; KCA4; potassium channel, calcium activated intermediate/small conductance subfamily N alpha, member 4; potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4; putative erythrocyte intermediate conductance calcium-activated potassium Gardos channel; putative Gardos channel; SK4; SKCa 4; SKCa4; small conductance calcium-activated potassium channel 4
  • Host
    Mouse
  • Isotype
    IgM
  • Protein IDO15554
  • Protein Name
    Intermediate conductance calcium-activated potassium channel protein 4
  • Scientific Description
    The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KCNN4 using anti-KCNN4 antibody (A01936-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysate, Lane 2: human PC-3 whole cell lysate, Lane 3: human A549 whole cell lysate, Lane 4: human Hela whole cell lysate, Lane 5: rat stomach tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNN4 antigen affinity purified polyclonal antibody (Catalog # A01936-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNN4 at approximately 48KD. The expected band size for KCNN4 is at 48KD.
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