![Wild-type (WT) and KLF5 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Wild-type (WT) and KLF5 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638321/GTX638321_T-44970_20230317_WB_KO_watermark_23032022_796.webp)
Wild-type (WT) and KLF5 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KLF5 antibody [HL2269]
GTX638321
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKLF5
Overview
- SupplierGeneTex
- Product NameKLF5 antibody [HL2269]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2269
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID688
- Target nameKLF5
- Target descriptionKLF transcription factor 5
- Target synonymsBTEB2, CKLF, IKLF, Krueppel-like factor 5, (intestinal Kruppel-like factor, BTE-binding protein 2, GC box binding protein 2, Kruppel like factor 5, Kruppel-like factor 5 (intestinal), basic transcription element binding protein 2, colon krueppel-like factor, colon kruppel-like factor, epididymis secretory sperm binding protein, intestinal-enriched krueppel-like factor, intestinal-enriched kruppel-like factor, transcription factor BTEB2
- HostRabbit
- IsotypeIgG
- Protein IDQ13887
- Protein NameKrueppel-like factor 5
- Scientific DescriptionThis gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638321/GTX638321_T-44970_20230317_WB_R_23032022_632.webp)
![KLF5 antibody [HL2269] detects KLF5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. KLF5 stained by KLF5 antibody [HL2269] (GTX638321) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min KLF5 antibody [HL2269] detects KLF5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. KLF5 stained by KLF5 antibody [HL2269] (GTX638321) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638321/GTX638321_T-44970_20230324_IHC-P_multiple_M_23032819_630.webp)
![Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638321/GTX638321_45033_20230505_WB_M_colon_23050918_337.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638321/GTX638321_45033_20230512_WB_23051702_406.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638321/GTX638321_45033_20230707_WB_B_23071223_563.webp)
![KLF5 antibody [HL2269] detects KLF5 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KLF5 stained by KLF5 antibody [HL2269] (GTX638321) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min KLF5 antibody [HL2269] detects KLF5 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KLF5 stained by KLF5 antibody [HL2269] (GTX638321) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638321/GTX638321_45033_20230829_IHC-P_M_23091319_123.webp)







