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ProductsBack/KRT7 / CK7 / Cytokeratin 7 Antibody (clone CK-7)

KRT7 / CK7 / Cytokeratin 7 Antibody (clone CK-7)

LS-C171105
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetKRT7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    KRT7 / CK7 / Cytokeratin 7 Antibody (clone CK-7)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-Fr, IHC-P, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CK-7
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3855
  • Target name
    KRT7
  • Target description
    keratin 7
  • Target synonyms
    CK7; CK-7; cytokeratin 7; K2C7; K7; keratin 7, type II; keratin, 55K type II cytoskeletal; keratin, simple epithelial type I, K7; keratin, type II cytoskeletal 7; sarcolectin; SCL; type II mesothelial keratin K7; type-II keratin Kb7
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KRT7 using anti-KRT7 antibody (A02416-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT7 antigen affinity purified polyclonal antibody (Catalog # A02416-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT7 at approximately 51 kDa. The expected band size for KRT7 is at 51 kDa.
Product group Antibodies
Boster Bio
Anti-KRT7 Antibody Picoband(r)A02416-2-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetKRT7
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Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CK7 Polyclonal Antibody(bs-1610R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss Antibodies
CK7 Polyclonal AntibodyBS-1610R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetKRT7
  • SizePrice
Product group Antibodies
Nordic-MUbio
Cytokeratin 7CK507
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetKRT7
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Product group Antibodies
Biomatik
KRT7 Polyclonal AntibodyCAC14736
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
TargetKRT7
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Product group Antibodies
Assay Genie
Cytokeratin 7 (KRT7) AntibodyCAB12004
TargetKRT7
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-Cytokeratin 7 [OV-TL 12/30]AB01648-1.1-BT
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetKRT7
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Immunohistochemistry analysis in human placenta and skeletal muscle tissues using AMAb91530 antibody. Corresponding KRT7 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-KRT7 AntibodyAMAB91530
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TargetKRT7
  • SizePrice
Product group Antibodies
RayBiotech
Anti-Cytokeratin 7 Antibody188-10504
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TargetKRT7
  • SizePrice
Product group Antibodies
Biosynth
Cytokeratin 7 antibody10R-C174A
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Product group Antibodies
Bethyl Laboratories
Rabbit anti-CK7 Recombinant Monoclonal Antibody [BC1]A700-186
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetKRT7
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