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ProductsBack/Rabbit anti-CK7 Recombinant Monoclonal Antibody [BC1]

Rabbit anti-CK7 Recombinant Monoclonal Antibody [BC1]

A700-186
Bethyl Laboratories
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetKRT7
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-CK7 Recombinant Monoclonal Antibody [BC1]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BC1
  • Concentration
    100 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3855
  • Target name
    KRT7
  • Target description
    keratin 7
  • Target synonyms
    CK7, K2C7, K7, SCL, keratin, type II cytoskeletal 7, CK-7, cytokeratin 7, keratin 7, type II, keratin, 55K type II cytoskeletal, keratin, simple epithelial type I, K7, sarcolectin, type II mesothelial keratin K7, type-II keratin Kb7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08729
  • Protein Name
    Keratin, type II cytoskeletal 7
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CK7 Polyclonal Antibody(bs-1610R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss
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ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetKRT7
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Product group Antibodies
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TargetKRT7
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Product group Antibodies
References
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1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200
Product group Antibodies
Cusabio
KRT7 Monoclonal AntibodyCSB-MA000210
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ReactivityHuman, Mouse, Rat
TargetKRT7
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Product group Antibodies
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TargetKRT7
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Product group Antibodies
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Figure 1. Western blot analysis of KRT7 using anti-KRT7 antibody (A02416-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT7 antigen affinity purified polyclonal antibody (Catalog # A02416-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT7 at approximately 51 kDa. The expected band size for KRT7 is at 51 kDa.
Product group Antibodies
Boster Bio
Anti-KRT7 Antibody Picoband(r)A02416-2-CARRIER-FREE
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TargetKRT7
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Product group Antibodies
Biosynth
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Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-CK7 Rabbit Monoclonal Antibody (Clone 284) at a 1:1000 dilution.
Product group Antibodies
RevMAb Biosciences
anti-Cytokeratin-7 (human), Rabbit Monoclonal (RM284)REV-31-1167-00
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TargetKRT7
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Immunohistochemistry analysis in human placenta and skeletal muscle tissues using AMAb91530 antibody. Corresponding KRT7 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
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TargetKRT7
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