Wild-type (WT) and Lamin B1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody [HL3224] (GTX640865) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody [HL3224] (GTX640865) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640865/GTX640865_T-45509_20240920_WB_M_R_24092600_447.webp "Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody [HL3224] (GTX640865) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody [HL3224] (GTX640865) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640865/GTX640865_T-45509_20240920_WB_TPM_watermark_24092600_682.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody [HL3224] (GTX640865) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody [HL3224] (GTX640865) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640865/GTX640865_T-45509_20241101_WB_treatment_Staurosporine_24110700_375.webp "Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody [HL3224] (GTX640865) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Lamin B1 antibody [HL3224] detects Lamin B1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Lamin B1 stained by Lamin B1 antibody [HL3224] (GTX640865) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min. Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640865/GTX640865_T-45509_20250117_IHC-P_multiple_RPKM_M_25012200_833.webp "Lamin B1 antibody [HL3224] detects Lamin B1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Lamin B1 stained by Lamin B1 antibody [HL3224] (GTX640865) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min. Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.")
![Lamin B1 antibody [HL3224] detects Lamin B1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Lamin B1 stained by Lamin B1 antibody [HL3224] (GTX640865) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640865/GTX640865_T-45509_20250117_IHC-P_M_25012200_216.webp "Lamin B1 antibody [HL3224] detects Lamin B1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Lamin B1 stained by Lamin B1 antibody [HL3224] (GTX640865) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Lamin B1 antibody [HL3224] detects Lamin B1 protein by immunohistochemical analysis. Sample: Paraffin-embedded human colon. Lamin B1 stained by Lamin B1 antibody [HL3224] (GTX640865) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640865/GTX640865_T-45509_20250117_IHC-P_25012200_948.webp "Lamin B1 antibody [HL3224] detects Lamin B1 protein by immunohistochemical analysis. Sample: Paraffin-embedded human colon. Lamin B1 stained by Lamin B1 antibody [HL3224] (GTX640865) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody [HL3224] (GTX640865) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640865/GTX640865_45593_20250801_WB_Fraction_25080802_391.webp "HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody [HL3224] (GTX640865) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Wild-type (WT) and Lamin B1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody [HL3224] (GTX640865) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Lamin B1 antibody [HL3224]
GTX640865
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLMNB1
Overview
- SupplierGeneTex
- Product NameLamin B1 antibody [HL3224]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3224
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4001
- Target nameLMNB1
- Target descriptionlamin B1
- Target synonymsADLD, ADLDAT, LMN, LMN2, LMNB, MCPH26, lamin-B1
- HostRabbit
- IsotypeIgG
- Protein IDP20700
- Protein NameLamin-B1
- Scientific DescriptionThis gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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