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ProductsBack/LIM1 antibody

LIM1 antibody

GTX129215
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetLHX1
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Overview

  • Supplier
    GeneTex
  • Product Name
    LIM1 antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.86 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3975
  • Target name
    LHX1
  • Target description
    LIM homeobox 1
  • Target synonyms
    LIM-1, LIM1, LIM/homeobox protein Lhx1, LIM homeobox protein 1, homeobox protein Lim-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48742
  • Protein Name
    LIM/homeobox protein Lhx1
  • Scientific Description
    This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and be involved in control of differentiation and development of neural and lymphoid cells. A similar protein in mice is an essential regulator of the vertebrate head organizer. [provided by RefSeq]
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Zhou M, Zhang M, Guo T, et al. Species origin of exogenous transcription factors affects the activation of endogenous pluripotency markers and signaling pathways of porcine induced pluripotent stem cells. Front Cell Dev Biol. 2023,11:1196273. doi: 10.3389/fcell.2023.1196273
    Read this paper

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Figure 1. Western blot analysis of LHX1 using anti-LHX1 antibody (A05816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LHX1 antigen affinity purified polyclonal antibody (Catalog # A05816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LHX1 at approximately 43KD. The expected band size for LHX1 is at 43KD.
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