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Immunohistochemistry of paraffin-embedded Human lung cancer using LRPPRC Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human lung cancer using LRPPRC Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human lung cancer using LRPPRC Polyclonal Antibody at dilution of 1:100 (40x lens).

LRPPRC Polyclonal Antibody

E-AB-62333
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    LRPPRC Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a leucine-rich protein that has multiple pentatricopeptide repeats (PPR). The precise role of this protein is unknown but studies suggest it may play a role in cytoskeletal organization, vesicular transport, or in transcriptional regulation of both nuclear and mitochondrial genes. The protein localizes primarily to mitochondria and is predicted to have an N-terminal mitochondrial targeting sequence. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome.
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate All lanes: LRPPRC antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 158 kDa Observed band size: 158 kDa
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Immunohistochemical staining of human cerebellum, colon, kidney and liver using Anti-LRPPRC antibody HPA036408 (A) shows similar protein distribution across tissues to independent antibody HPA036409 (B).
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Figure 1. Western blot analysis of LRPPRC using anti-LRPPRC antibody (A03264). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: monkey kidney tissue lysates, Lane 6: monkey heart tissue lysates, Lane 7: rat heart tissue lysates, Lane 8: rat skeletal muscle tissue lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat liver tissue lysates, Lane 11: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRPPRC antigen affinity purified polyclonal antibody (Catalog # A03264) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRPPRC at approximately 157KD. The expected band size for LRPPRC is at 150-160KD.
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