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ProductsBack/Macrophage mannose receptor 1 Antibody (YA3531, Rabbit)

Macrophage mannose receptor 1 Antibody (YA3531, Rabbit)

HY-P81214
MedChem Express
TargetMRC1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Macrophage mannose receptor 1 Antibody (YA3531, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4360
  • Target name
    MRC1
  • Target description
    mannose receptor C-type 1
  • Target synonyms
    CD206, CLEC13D, CLEC13DL, MMR, MRC1L1, bA541I19.1, hMR, macrophage mannose receptor 1, C-type lectin domain family 13 member D, human mannose receptor, macrophage mannose receptor 1-like protein 1, mannose receptor, C type 1-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    MRC1 Antibody is an unconjugated, approximately 160 kDa, rabbit-derived, anti-MRC1 monoclonal antibody. MRC1 Antibody can be used for: WB expriments in human and rat background without labeling.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MRC1 using anti-MRC1 antibody (A02285-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: human liver tissue lysates, Lane 4: monkey lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRC1 antigen affinity purified polyclonal antibody (Catalog # A02285-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRC1 at approximately 190-200 kDa. The expected band size for MRC1 is at 166 kDa.
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HepG2 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MRC1 Antibody（bs-4727R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
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