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Western blot analysis of extracts from HeLa/COS7/RAW246.7 cells, using Tau (Ab-235) antibody.
Western blot analysis of extracts from HeLa/COS7/RAW246.7 cells, using Tau (Ab-235) antibody.
Western blot analysis of extracts from HeLa/COS7/RAW246.7 cells, using Tau (Ab-235) antibody.

MAPT (Ab-235) Antibody

CSB-PA795646
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAPT
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Overview

  • Supplier
    Cusabio
  • Product Name
    MAPT (Ab-235) Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4137
  • Target name
    MAPT
  • Target description
    microtubule associated protein tau
  • Target synonyms
    DDPAC; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; MAPTL; microtubule-associated protein tau; MSTD; MTBT1; MTBT2; neurofibrillary tangle protein; paired helical filament-tau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; TAU; tau-40
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10636
  • Protein Name
    Microtubule-associated protein tau
  • Scientific Description
    Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. Alonso Adel C, et al. (2004) J Biol Chem. 279(33): 34873-34881. Liu F, et al. (2002) FEBS Lett. 530(1-3): 209-214. Sengupta A, et al. (1998) Arch Biochem Biophys. 357(2): 299-309.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tTau protein Polyclonal Antibody, Unconjugated (bs-0157R) at 1:200 overnight at 4°C, followed by a conjugated secondary Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml) was used to stain the cell nuclei.
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Figure 1. Western blot analysis of Tau/MAPT using anti-Tau/MAPT antibody (A00097-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tau/MAPT antigen affinity purified polyclonal antibody (Catalog # A00097-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tau/MAPT at approximately 50-70 kDa. The expected band size for Tau/MAPT is at 79 kDa.
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