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Thioester Activity Assay: UBE2K forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of excess DTT. The thioester assay also shows di-ubiquitin formation with addition of ATP. This confirms the activity of
Product group Proteins / Signaling Molecules
South Bay Bio
UBE2K [UbcH1, E2-25K] (human) (rec.) (His)SBB-CE0022
Protein IDP61086
  • SizePrice
Thioester Activity Assay: UBE2D2 (UbcH5b) forms a thioester with UB in an ATP dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D2.
Product group Proteins / Signaling Molecules
South Bay Bio
UBE2D2 [UbcH5b] (human) (rec.) (untagged)SBB-CE0027
Protein IDP62837
  • SizePrice
Michaelis-Menten Kinetics: NEDD8 Rhodamine 110 serially diluted from 1.6 to 0.0125microM was digested with 30pM NEDP1 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 1mM TCEP, 0.1 mg/ml BSA, at 25°C. Initial velocities a
Product group Proteins / Signaling Molecules
South Bay Bio
NEDP1 [SENP8] (human) (rec.) (His)SBB-DE0025
Protein IDQ96LD8
  • SizePrice
Product group Proteins / Signaling Molecules
South Bay Bio
SENP1 (catalytic domain) (human) (rec.) (His)SBB-DE0026
Protein IDQ9P0U3
  • SizePrice
% Signal to Background of Continuous Real-Time TR-FRET NEDD4 titration (autoubiquitination): Serial dilutions of NEDD4 from 50nM to 3.125nM mixed with UBA1, UBE2L3, and, TRF-Ub mix. Reaction was initiated with addition of Mg-ATP.
Product group Chemicals
South Bay Bio
XIAP E3 Ligase TR-FRET KitSBB-KF0057
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20S Immunoproteasome vs. 20S Constitutive Proteasome Activity: 20S immunoproteasome is most active against Suc-LLVY-AMC (SBB-PS0010), Ac-PAL-AMC (SBB-PS0007), and Ac-ANW-AMC (SBB-PS0009) substrates, representing physiologically relevant chymotrypsi
Product group Proteins / Signaling Molecules
South Bay Bio
20S Immunoproteasome (human) (untagged)SBB-PP0004
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20S Immunoproteasome vs. 20S Constitutive Proteasome Activity: 20S Proteasome is most active against Suc-LLVY-AMC (SBB-PS0010), Z-LLE-AMC (SBB-PS0006), and Ac-WLA-AMC (SBB-PS0008) substrates, representing physiologically relevant chymotry
Product group Proteins / Signaling Molecules
South Bay Bio
20S Proteasome (human) (untagged)SBB-PP0005
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20S Immunoproteasome vs. 20S Constitutive Proteasome Activity: 20S immunoproteasome is most active against Suc-LLVY-AMC (SBB-PS0010), Ac-PAL-AMC (SBB-PS0007), and Ac-ANW-AMC (SBB-PS0009) substrates, representing physiologically relevant chymotrypsin-like, beta1i, and beta5i immunoproteasome activity respectively.
Product group Proteins / Signaling Molecules
South Bay Bio
20S Immunoproteasome (human) (untagged)SBB-PP0144
  • SizePrice
Chemical Structure
Product group Assays
South Bay Bio
Ac-Pro-Ala-Leu-AMC [Ac-PAL-AMC]SBB-PS0007
  • SizePrice
Chemical Structure
Product group Assays
South Bay Bio
Ac-Trp-Leu-Ala-AMC [Ac-WLA-AMC]SBB-PS0008
  • SizePrice
Chemical Structure
Product group Assays
South Bay Bio
Suc-Leu-Leu-Val-Tyr-AMC [Suc-LLVY-AMC]SBB-PS0010
  • SizePrice
HA-Ubiquitin-vinyl sulfone (human) (rec.) (HA)
Product group Proteins / Signaling Molecules
South Bay Bio
HA-Ubiquitin-vinyl sulfone (human) (rec.) (HA)SBB-PS0122
Protein IDP0CG47
  • SizePrice
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