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Western blot analysis of T-bet using anti-T-bet antibody (PA1999). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-T-bet antigen affinity purified polyclonal antibody (Catalog # PA1999) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for T-bet at approximately 58-60KD. The expected band size for T-bet is at 58KD.
Product group Antibodies
Boster Bio
Anti-T-bet/Tbx21 Antibody Picoband(r)PA1999
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetTBX21
  • SizePrice
Anti-Tec antibody, PA2000, Western blotting Lane 1: Rat Liver Tissue Lysate Lane 2: Rat Spleen Tissue Lysate Lane 3: Rat Kidney Tissue Lysate Lane 4: HELA Cell Lysate Lane 5: JURKAT Cell Lysate
Product group Antibodies
Boster Bio
Anti-Tyrosine-protein kinase Tec Tec Antibody Picoband(r)PA2000
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Rat
TargetTEC
  • SizePrice
Anti-TRIF antibody, PA2001, Western blotting Recombinant Protein Detection Source: E.coli derived -recombinant Mouse TICAM1,37.5KD
Product group Antibodies
Boster Bio
Anti-TRIF/TICAM1 Antibody Picoband(r)PA2001
ApplicationsWestern Blot
ReactivityMouse
TargetTicam1
  • SizePrice
Anti-DR3 antibody, PA2004, Western blotting All lanes: Anti DR3 (PA2004) at 0.5ug/ml WB: COLO320 Whole Cell Lysate at 40ug Predicted bind size: 59KD Observed bind size: 59KD
Product group Antibodies
Boster Bio
Anti-DR3/TNFRSF25 Antibody Picoband(r)PA2004
ApplicationsWestern Blot
ReactivityHuman
TargetTNFRSF25
  • SizePrice
Western blot analysis of Tollip using anti-Tollip antibody (PA2005).
Product group Antibodies
Boster Bio
Anti-Toll-interacting protein Tollip Antibody Picoband(r)PA2005
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityBovine, Human
TargetTOLLIP
  • SizePrice
Western blot analysis of TRAF1 using anti-TRAF1 antibody (PA2006). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human T-47D whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF1 antigen affinity purified polyclonal antibody (Catalog # PA2006) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF1 at approximately 46KD. The expected band size for TRAF1 is at 46KD.
Product group Antibodies
Boster Bio
Anti-TRAF1 Antibody Picoband(r)PA2006
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse
TargetTRAF1
  • SizePrice
Anti-TRAF2 antibody, PA2007, IHC(P) IHC(P): Human Tonsil Tissue
Product group Antibodies
Boster Bio
Anti-TRAF2 Antibody Picoband(r)PA2007
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetTRAF2
  • SizePrice
Anti-TRAF3 antibody, PA2008, Western blotting Recombinant Protein Detection Source: E.coli derived -recombinant Human TRAF3, 30.6KD
Product group Antibodies
Boster Bio
Anti-TRAF3 Antibody Picoband(r)PA2008
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetTRAF3
  • SizePrice
Anti-TRAF4 antibody, PA2009, Western blotting Lane 1: Rat Thymus Tissue Lysate Lane 2: HELA Cell Lysate Lane 3: JURKAT Cell Lysate Lane 4: HEPA Cell Lysate
Product group Antibodies
Boster Bio
Anti-TRAF4 Antibody Picoband(r)PA2009
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetTRAF4
  • SizePrice
Anti-TRAM1 antibody, PA2010, IHC(P) IHC(P): Human Lung Cancer Tissue
Product group Antibodies
Boster Bio
Anti-TRAM1 Antibody Picoband(r)PA2010
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHamster, Human, Mouse, Rat
TargetTRAM1
  • SizePrice
Anti-TRAM2 antibody, PA2011, Western blotting Recombinant Protein Detection Source: E.coli derived -recombinant Mouse TRAM2,39.0KD
Product group Antibodies
Boster Bio
Anti-TRAM2 Antibody Picoband(r)PA2011
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetTram2
  • SizePrice
Anti-Hamartin antibody, PA2012, Western blotting All lanes: Anti Hamartin (PA2012) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug Lane 2: Rat Heart Tissue Lysate at 50ug Lane 3: Rat Brain Tissue Lysate at 50ug Lane 4: Rat Lung Tissue Lysate at 50ug Lane 5: 293T Whole Cell Lysate at 40ug Lane 6: HELA Whole Cell Lysate at 40ug Lane 7: HT1080 Whole Cell Lysate at 40ug Lane 8: SMMC Whole Cell Lysate at 40ug Predicted bind size: 130KD Observed bind size: 160KD
Product group Antibodies
Boster Bio
Anti-Hamartin/TSC1 Antibody Picoband(r)PA2012
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetTSC1
  • SizePrice
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