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IHC analysis of C Reactive Protein using anti-C Reactive Protein antibody (A00249-4). C Reactive Protein was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-C Reactive Protein Antibody (A00249-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-C Reactive Protein/CRP AntibodyA00249-4
Western blot analysis of Ace using anti-Ace antibody (A00251).
Product group Antibodies
Boster Bio
Anti-Angiotensin Converting Enzyme 1/Ace AntibodyA00251
Western blot analysis of CARS using anti-CARS antibody (A00259-1).
Product group Antibodies
Boster Bio
Anti-CARS AntibodyA00259-1
Western blot analysis of BMAL1/ARNTL using anti-BMAL1/ARNTL antibody (A00260-1).
Product group Antibodies
Boster Bio
Anti-BMAL1/ARNTL AntibodyA00260-1
Western blot analysis of FOXA1 using anti-FOXA1 antibody (A00266-1).
Product group Antibodies
Boster Bio
Anti-FOXA1 AntibodyA00266-1
IHC analysis of AGRP using anti-AGRP antibody (A00272-1).
Product group Antibodies
Boster Bio
Anti-AGRP AntibodyA00272-1
Western blot analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MDA-MB-231 whole cell lysates, Lane 5: human PANC-1 whole cell lysates, Lane 6: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-kB p65 antigen affinity purified polyclonal antibody (Catalog # A00284-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF-kB p65 at approximately 65KD. The expected band size for NF-kB p65 is at 65KD.
Product group Antibodies
Boster Bio
Anti-NF-kB p65/RELA AntibodyA00284-1
Western blot analysis of MMP2 using anti-MMP2 antibody (A00286). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human MMP2 protein 1ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP2 antigen affinity purified polyclonal antibody (Catalog # A00286) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP2 at approximately 71KD. The expected band size for MMP2 is at 71KD.
Product group Antibodies
Boster Bio
Anti-MMP2 Picoband AntibodyA00286
IHC analysis of ICOS using anti-ICOS antibody (A00291-2).
Product group Antibodies
Boster Bio
Anti-ICOS AntibodyA00291-2
IHC analysis of ICOS using anti-ICOS antibody (A00291-3).
Product group Antibodies
Boster Bio
Anti-ICOS AntibodyA00291-3
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human ICOS DyLight 488 conjugated AntibodyA00291-Dyl488
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human ICOS DyLight 550 conjugated AntibodyA00291-Dyl550
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