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Western blot analysis of NOX2 using anti-NOX2 antibody (A00328). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG cell lysate, Lane 2: human HeLa cell lysate, Lane 3: human HepG2 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX2 antigen affinity purified polyclonal antibody (Catalog # A00328) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOX2 at approximately 65KD. The expected band size for NOX2 is at 65KD.
Product group Antibodies
Boster Bio
Anti-NOX2/gp91phox/CYBB AntibodyA00328
Western blot analysis of JAK1 using anti-JAK1 antibody (A00330).
Product group Antibodies
Boster Bio
Anti-JAK1 Picoband AntibodyA00330
IHC analysis of Calpastatin using anti-Calpastatin antibody (A00337). Calpastatin was detected in paraffin-embedded section of human intetsinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calpastatin Antibody (A00337) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Calpastatin Picoband AntibodyA00337
Western blot analysis of NFAT2 using anti-NFAT2 antibody (A00340-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT2 antigen affinity purified polyclonal antibody (Catalog # A00340-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT2 at approximately 101KD. The expected band size for NFAT2 is at 101KD.
Product group Antibodies
Boster Bio
Anti-NFAT2 AntibodyA00340-1
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human NFAT2 DyLight 488 conjugated AntibodyA00340-Dyl488
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human NFAT2 DyLight 550 conjugated AntibodyA00340-Dyl550
Western blot analysis of NGF/NGF Beta using anti-NGF/NGF Beta antibody (A00341).
Product group Antibodies
Boster Bio
Anti-NGF/NGF Beta AntibodyA00341
Western blot analysis of BAP1 using anti-BAP1 antibody (A00345-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 22RV1 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAP1 antigen affinity purified polyclonal antibody (Catalog # A00345-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAP1 at approximately 88KD. The expected band size for BAP1 is at 80KD.
Product group Antibodies
Boster Bio
Anti-BAP1 AntibodyA00345-1
Western blot analysis of ATG7 using anti-ATG7 antibody (A00346).
Product group Antibodies
Boster Bio
Anti-Apg7 AntibodyA00346
Western blot analysis of PDGF beta using anti-PDGF beta antibody (A00348). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGF beta antigen affinity purified polyclonal antibody (Catalog # A00348) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDGF beta at approximately 27KD. The expected band size for PDGF beta is at 27KD.
Product group Antibodies
Boster Bio
Anti-PDGF beta AntibodyA00348
IHC analysis of Leptin Receptor/LEPR using anti-Leptin Receptor/LEPR antibody (A00350).
Product group Antibodies
Boster Bio
Anti-Leptin Receptor AntibodyA00350
Western blot analysis of Granzyme B using anti-Granzyme B antibody (A00353-1).
Product group Antibodies
Boster Bio
Anti-Granzyme B/Gzmb AntibodyA00353-1
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