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Detection of endogenous fer: Whole cell extracts of pervanadate (VH) treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab fer-6B9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). VH treatment: 15 min. Lane 1: A431; Lane 2: SW480 lane 3: SW620; lane 4: HT29; lane 5: MCF-7; lane 6: MDA-MB231; lane 7: T47D
Product group Antibodies
OriGene
FER (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6B9]AM00060PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetFER
  • SizePrice
fos activation Serum starved HepG2 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). Immunoblots were probed with mab fos-34E4 (0.5 ug/ ml) for 1h at room temperature and developed by ECL (exp. time: 30 sec). lane 1: control; lane 2: 5 min EGF; lane 3: 15 min EGF; lane 4: 30 min EGF; lane 5: 1h EGF;lane 6; 2h EGF lane 7: 4h EGF; lane 8: 8h EGF
Product group Antibodies
OriGene
c Fos (FOS) pSer374 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 34E4]AM00061PU-N
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human
TargetFOS
  • SizePrice
Detection of endogenous FOS: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab FOS-8B5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: A549 Lane 3: SKOV3 Lane 4: OVCAR5 Lane 5: HaCaT Lane 6: PC3 Lane 7: HeLa Lane 8: HepG2
Product group Antibodies
OriGene
c Fos (FOS) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 8B5]AM00062PU-N
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetFOS
  • SizePrice
Product group Antibodies
OriGene
N-Acetylglucosamine (GlcNAc) Mouse Monoclonal Antibody [Clone ID: 10D8]AM00063PU-N
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
  • SizePrice
Detection of endogenous GSK3alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab GSK3a-10C1 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: A549 Lane 3: SKOV-3 Lane 4: OVCAR-5 Lane 5: HaCaT Lane 6: PC3M Lane 7: Hela Lane 8: HepG2
Product group Antibodies
OriGene
GSK3 alpha (GSK3A) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10C1]AM00064PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse, Rat
TargetGSK3A
  • SizePrice
Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A431 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with mab GSK-9B8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
GSK3 alpha (GSK3A) pSer21 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9B8]AM00065PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetGSK3A
  • SizePrice
Product group Antibodies
OriGene
GSK3 beta (GSK3B) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11B9]AM00066BT-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetGSK3B
  • SizePrice
Detection of Endogenous GSK3 beta: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab GSK3-11B9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2:SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF7 Lane 6: MDA-MB-231 Lane 7: T47D
Product group Antibodies
OriGene
GSK3 beta (GSK3B) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11B9]AM00066PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetGSK3B
  • SizePrice
Product group Antibodies
OriGene
GSK3 beta (GSK3B) pSer9 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 3A8]AM00067BT-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse
TargetGSK3B
  • SizePrice
Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A431 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with mab GSK-3A8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
GSK3 beta (GSK3B) pSer9 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 3A8]AM00067PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse
TargetGSK3B
  • SizePrice
Phosphospecificity Whole cell extracts of control (co) and pervanadate (VH) treated A431 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with mab GSK3b-2D3 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
GSK3 beta (GSK3B) pSer9 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2D3]AM00068PU-N
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetGSK3B
  • SizePrice
Antibody sensitivity Recombinant GST-PIN1 was separated by SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab 4H3 (0.5 g/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: 100ng, lane 2: 50ng, lane 3: 25ng, lane 4
Product group Antibodies
OriGene
GST-Tag Mouse Monoclonal Antibody [Clone ID: 4H3]AM00069PU-N
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivitySchistosoma japonicum
  • SizePrice
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