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Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-9H2 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
EGFR pTyr1173 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9H2]AM00042PU-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse
TargetEGFR
  • SizePrice
Product group Antibodies
OriGene
EGFR Non pTyr1197 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 20G3]AM00044BT-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse
TargetEGFR
  • SizePrice
Phosphospecificity Whole cell extracts of control (co) and EGF stimulated (EGF) A431 tumor cells were applied to SDS-PAGE (ca. 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-10G12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
EGFR Non pTyr1197 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 20G3]AM00044PU-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse
TargetEGFR
  • SizePrice
Product group Antibodies
OriGene
EGFR (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 14C8]AM00045BT-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse
TargetEGFR
  • SizePrice
Detection of endogenous EGFR: Whole cell lysates of EGF stimulated serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-14C8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Product group Antibodies
OriGene
EGFR (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 14C8]AM00045PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse
TargetEGFR
  • SizePrice
Product group Antibodies
OriGene
EGFR (Extracell. Dom.) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 20E12]AM00046BT-N
ApplicationsWestern Blot
ReactivityHuman
TargetEGFR
  • SizePrice
Antibody sensitivity Whole cell lysates of A431cells containing defined cell numbers per lane were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-20E12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: 160.000 cells, lane 2: 80.000 cells lane 3: 40.000 cells, lane 4: 20.000 cells, lane 5: 10.000 cells, lane 6: 5.000 cells, lane 7: 2.500 cells, lane 8: 1.000 cells
Product group Antibodies
OriGene
EGFR (Extracell. Dom.) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 20E12]AM00046PU-N
ApplicationsWestern Blot
ReactivityHuman
TargetEGFR
  • SizePrice
Product group Antibodies
OriGene
EGFR (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 13G8]AM00047BT-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse
TargetEGFR
  • SizePrice
Detection of endogenous EGFR: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-13G8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Product group Antibodies
OriGene
EGFR (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 13G8]AM00047PU-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse
TargetEGFR
  • SizePrice
ERBB2 Transactivation: Serum starved A431 cells were treated for 15 min as indicated. Whole cell lysates were separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2-7F8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control Lane 2: PMA Lane 3: LPA Lane 4: Ceramide Lane 5: Bradykinin Lane 6: Bombesin
Product group Antibodies
OriGene
Her2 (ERBB2) pThr686 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7F8]AM00048PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse
TargetERBB2
  • SizePrice
Detection of endogenous erbB2 Whole cell extracts of serum starved, EGF-treated (15 min) tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB2-24B5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2A 549 Lane 3: SKOV3 Lane 4: OVCAR5 Lane 5: HaCaT Lane 6: PC3 Lane 7: HeLa Lane 8: HepG2
Product group Antibodies
OriGene
Her2 (ERBB2) (860-880) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 24B5]AM00049PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse
TargetERBB2
  • SizePrice
Product group Antibodies
OriGene
Her2 (ERBB2) pTyr1112 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 19G5]AM00050BT-N
ApplicationsImmunoFluorescence, Western Blot
ReactivityCanine, Human, Mouse, Rat
TargetERBB2
  • SizePrice
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