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Western blot analysis of Phospho-Paxillin (Y118) expression in (1) HeLa cell lysate; (2) HeLa cell treated with pervanadate.
Product group Antibodies
Boster Bio
Anti-Phospho-Paxillin (Y118) PXN Rabbit Monoclonal AntibodyP01033
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPXN
Western blot analysis of Phospho-RSK1(S380) expression in A431 cell lysate treated with EGF (P01058-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KA1 monoclonal antibody (Catalog # P01058-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS6KA1
Product group Antibodies
Boster Bio
Anti-Phospho-RSK1 (S380) RPS6KA1 Rabbit Monoclonal AntibodyP01058-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetRPS6KA1
Western blot analysis of Phospho-Chk1 (S296) expression in HEK293 cell lysate Treated with Calyculin (P01060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK1 monoclonal antibody (Catalog # P01060) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHEK1
Product group Antibodies
Boster Bio
Anti-Phospho-Chk1 (S296) CHEK1 Rabbit Monoclonal AntibodyP01060
ApplicationsWestern Blot
ReactivityHuman
TargetCHEK1
Western blot analysis of Phospho-Stat5 (Y694) expression in A431 cell lysate treated with EGF (P01087-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A monoclonal antibody (Catalog # P01087-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT5A
Product group Antibodies
References
Boster Bio
Anti-Phospho-Stat5 (Y694) STAT5A Rabbit Monoclonal AntibodyP01087-1
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetSTAT5A
Western blot analysis of Phospho-IKB alpha (S32) expression in HeLa cell lysate treated with Calyculin A and TNF-a (P01139). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFKBIA monoclonal antibody (Catalog # P01139) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
References
Boster Bio
Anti-Phospho-IKB alpha (S32) NFKBIA Rabbit Monoclonal AntibodyP01139
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetNFKBIA
Western blot analysis of Phospho-GCN2 (Thr899) in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Calyculin.
Product group Antibodies
Boster Bio
Anti-Phospho-GCN2 (T899) EIF2AK4 Rabbit Monoclonal AntibodyP01172
ApplicationsWestern Blot
ReactivityHuman
TargetEIF2AK4
Western blot analysis of Phospho-Cdc6 (S54) expression in (1) Raji cell lysate; (2) Raji + FBS cell lysate.
Product group Antibodies
Boster Bio
Anti-Phospho-Cdc6 (S54) Rabbit Monoclonal AntibodyP01355
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetCDC6
Western blot analysis of PKR phosphorylation expression in HeLa cell lysate treated with Calyculin A and TNF-alpha.
Product group Antibodies
Boster Bio
Anti-Phospho-PKR (T446) EIF2AK2 Rabbit Monoclonal AntibodyP01384
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetEIF2AK2
Western blot analysis of Phospho-TrkB (Y817) expression in SH-SY5Y cell lysate treated with BDNF.
Product group Antibodies
Boster Bio
Anti-Phospho-TrkB (Y817) NTRK2 Rabbit Monoclonal AntibodyP01388
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNTRK2
Western blot analysis of AMPK beta 1 expression in (1) 293T cell lysate treated with Lambda lysate; (2) 293T cell lysate (P01420-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAA2 monoclonal antibody (Catalog # P01420-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKAA2
Product group Antibodies
Boster Bio
Anti-Phospho-AMPK alpha 2 (S491) PRKAA2 Rabbit Monoclonal AntibodyP01420-1
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPRKAA2
Western blot analysis of Phospho-AMPK alpha (T172) expression in Mouse heart lysate.
Product group Antibodies
Boster Bio
Anti-Phospho-AMPK alpha (T172) PRKAA2 Rabbit Monoclonal AntibodyP01420-2
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPRKAA1
Western blot analysis of Phospho-AMPK alpha 2 (S345) expression in (1) 293T cell lysate treated with Lambda Phosphatase; (2) 293T cell lysate (P01420). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAA2 monoclonal antibody (Catalog # P01420) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKAA2
Product group Antibodies
Boster Bio
Anti-Phospho-AMPK alpha 2 (S345) PRKAA2 Rabbit Monoclonal AntibodyP01420
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPRKAA2
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