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Western blot analysis of CD14 expression in Human tonsil cell lysate (M00137). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD14 monoclonal antibody (Catalog # M00137) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD14
Product group Antibodies
Boster Bio
Anti-CD14 Rabbit Monoclonal AntibodyM00137
Western blot analysis of Presenilin 1 expression in Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-Presenilin 1 Monoclonal AntibodyM00138-1
Immunohistochemical analysis of paraffin-embedded human colon cancer, using MMP9 Antibody.
Product group Antibodies
Boster Bio
Anti-MMP9 Rabbit Monoclonal AntibodyM00139
Western blot analysis of MSH2 expression in HeLa cell lysate using anti-MSH2 antibody (M00140). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSH2 antigen affinity purified polyclonal antibody (Catalog # M00140) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MSH2.
Product group Antibodies
Boster Bio
Anti-MSH2 Monoclonal AntibodyM00140
Western blot analysis of RIP expression in HeLa cell lysate (M00141). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIPK1 monoclonal antibody (Catalog # M00141) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIPK1
Product group Antibodies
Boster Bio
Anti-RIP Rabbit Monoclonal AntibodyM00141
Immunohistochemical analysis of paraffin-embedded human tonsil, using Bcl-6 Antibody(M00142-1) BCL6 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BCL6 Antibody (M00142-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Bcl-6 Rabbit Monoclonal AntibodyM00142-1
Western blot analysis of CA2 using anti-CA2 antibody (M00143).
Product group Antibodies
Boster Bio
Anti-CA2 Antibody (monoclonal, 10E11)M00143
Immunohistochemical analysis of paraffin-embedded human spleen, using CD11b Antibody.
Product group Antibodies
Boster Bio
Anti-CD11b Rabbit Monoclonal AntibodyM00144-1
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CD11b Antibody(M00144) ITGAM was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ITGAM Antibody (M00144)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-CD11b Rabbit Monoclonal AntibodyM00144
Anti-p21 Rabbit Monoclonal Antibody
Product group Antibodies
Boster Bio
Anti-p21 Rabbit Monoclonal AntibodyM00145-2
Immunohistochemical analysis of paraffin-embedded human colon, using p21 Antibody(M00145-3) CDKN1A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDKN1A Antibody (M00145-3)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-p21 Rabbit Monoclonal AntibodyM00145-3
Western blot analysis of Calreticulin expression in IGF1 recombinant protein (M00148). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF1 monoclonal antibody (Catalog # M00148) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-IGF1/Igf I Rabbit Monoclonal AntibodyM00148
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