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Western blot analysis of CXCR4 using anti-CXCR4 antibody (A00031).
Product group Antibodies
Boster Bio
Anti-CXCR4 AntibodyA00031
Western blot analysis of Notch1 using anti-Notch1 antibody (A00033-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse skeletal muscle tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Notch1 antigen affinity purified polyclonal antibody (Catalog # A00033-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Notch1 at approximately 272KD. The expected band size for Notch1 is at 272KD.
Product group Antibodies
Boster Bio
Anti-Notch1 AntibodyA00033-2
IHC analysis of STAT1 using anti-STAT1 antibody (A00036-2). STAT1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-STAT1 Antibody (A00036-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-STAT1 AntibodyA00036-2
Western blot analysis of BCL2 using anti-BCL2 antibody (A00040-2).
Product group Antibodies
Boster Bio
Anti-Bcl-2/BCL2 AntibodyA00040-2
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human Bcl-2 DyLight 488 conjugated AntibodyA00040-Dyl488
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human Bcl-2 DyLight 550 conjugated AntibodyA00040-Dyl550
IHC analysis of Prothrombin using anti-Prothrombin antibody (A00044).
Product group Antibodies
Boster Bio
Anti-Prothrombin/F2 AntibodyA00044
Western blot analysis of VEGF/VEGF164 using anti-VEGF/VEGF164 antibody (A00045-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse HEPA1-6 whole cell lysates, Lane 3: rat C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF/VEGF164 antigen affinity purified polyclonal antibody (Catalog # A00045-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF/VEGF164 at approximately 27KD, 40KD. The expected band size for VEGF/VEGF164 is at 25KD.
Product group Antibodies
Boster Bio
Anti-VEGF/Vegfa AntibodyA00045-1
Western blot analysis of VEGF using anti-VEGF antibody (A00045).
Product group Antibodies
Boster Bio
Anti-VEGF AntibodyA00045
Western blot analysis of P Glycoprotein using anti-P Glycoprotein antibody (A00049-1).
Product group Antibodies
Boster Bio
Anti-P Glycoprotein/ABCB1 AntibodyA00049-1
Western blot analysis of StAR using anti-StAR antibody (A00051-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-StAR antigen affinity purified polyclonal antibody (Catalog # A00051-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for StAR at approximately 25KD. The expected band size for StAR is at 32KD.
Product group Antibodies
Boster Bio
Anti-StAR AntibodyA00051-1
IHC analysis of CD44 using anti-CD44 antibody (A00052).
Product group Antibodies
Boster Bio
Anti-CD44 AntibodyA00052
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