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Western blot All lanes: MFN1 antibody at 3.78microg/ml Lane 1: Human placenta tissue Lane 2: Mouse kidney tissue Lane 3: NIH/3T3 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: Hela whole cell lysate Lane 6: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 85, 42, 72 kDa Observed band size: 85 kDa
Western blot All lanes: MFN1 antibody at 3.78microg/ml Lane 1: Human placenta tissue Lane 2: Mouse kidney tissue Lane 3: NIH/3T3 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: Hela whole cell lysate Lane 6: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 85, 42, 72 kDa Observed band size: 85 kDa
Western blot All lanes: MFN1 antibody at 3.78microg/ml Lane 1: Human placenta tissue Lane 2: Mouse kidney tissue Lane 3: NIH/3T3 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: Hela whole cell lysate Lane 6: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 85, 42, 72 kDa Observed band size: 85 kDa

MFN1 Antibody

CSB-PA812879ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMFN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    MFN1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55669
  • Target name
    MFN1
  • Target description
    mitofusin 1
  • Target synonyms
    fzo homolog; hfzo1; hfzo2; mitochondrial transmembrane GTPase Fzo-1; mitochondrial transmembrane GTPase FZO-2; mitofusin-1; putative transmembrane GTPase; transmembrane GTPase MFN1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IWA4
  • Protein Name
    Mitofusin-1
  • Scientific Description
    Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Mitofusin 1 using anti-Mitofusin 1 antibody (PB9264). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofusin 1 antigen affinity purified polyclonal antibody (Catalog # PB9264) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofusin 1 at approximately 84KD. The expected band size for Mitofusin 1 is at 84KD.
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