![Boiled and unboiled human tissue extract (5 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3509] (GTX641397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Boiled and unboiled human tissue extract (5 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3509] (GTX641397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641397/GTX641397_T-45607_20250110_WB_brain_ub_25011618_281.webp)
Boiled and unboiled human tissue extract (5 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3509] (GTX641397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
mGluR5 antibody [HL3509]
GTX641397
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGRM5
Overview
- SupplierGeneTex
- Product NamemGluR5 antibody [HL3509]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3509
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2915
- Target nameGRM5
- Target descriptionglutamate metabotropic receptor 5
- Target synonymsGPRC1E, MGLUR5, PPP1R86, mGlu5, metabotropic glutamate receptor 5, glutamate receptor, metabotropic 5, protein phosphatase 1, regulatory subunit 86
- HostRabbit
- IsotypeIgG
- Protein IDP41594
- Protein NameMetabotropic glutamate receptor 5
- Scientific DescriptionThis gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3509] (GTX641397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3509] (GTX641397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641397/GTX641397_T-45607_20250110_WB_M_tissue_25011618_868.webp)
![Unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3509] (GTX641397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3509] (GTX641397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641397/GTX641397_T-45607_20250110_WB_R_tissue_25011618_503.webp)
![mGluR5 antibody [HL3509] detects mGluR5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain (striatum). mGluR5 stained by mGluR5 antibody [HL3509] (GTX641397) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min mGluR5 antibody [HL3509] detects mGluR5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain (striatum). mGluR5 stained by mGluR5 antibody [HL3509] (GTX641397) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX641397/GTX641397_T-45607_20250127_IHC-P_M_25021923_962.webp)
![mGluR5 antibody [HL3509] detects mGluR5 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain (hippocampus). mGluR5 stained by mGluR5 antibody [HL3509] (GTX641397) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min mGluR5 antibody [HL3509] detects mGluR5 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain (hippocampus). mGluR5 stained by mGluR5 antibody [HL3509] (GTX641397) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX641397/GTX641397_T-45607_20250127_IHC-P_R_25021923_143.webp)
![mGluR5 antibody [HL3509] detects mGluR5 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse striatum. Green: mGluR5 stained by mGluR5 antibody [HL3509] (GTX641397) diluted at 1:250. Red: NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Hoechst 33342 staining. mGluR5 antibody [HL3509] detects mGluR5 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse striatum. Green: mGluR5 stained by mGluR5 antibody [HL3509] (GTX641397) diluted at 1:250. Red: NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641397/GTX641397_T-45607_20250328_IHC-Fr_M_25042420_293.webp)
![mGluR5 antibody [HL3509] detects mGluR5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampus. mGluR5 stained by mGluR5 antibody [HL3509] (GTX641397) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min mGluR5 antibody [HL3509] detects mGluR5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampus. mGluR5 stained by mGluR5 antibody [HL3509] (GTX641397) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX641397/GTX641397_T-45607_20250328_IHC-P_M_25051420_764.webp)



![Boiled and unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL2543] (GTX638912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638912/GTX638912_T-45124_20230818_WB_M_R_23082201_444.webp)
![Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL2876] (GTX640153) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640153/GTX640153_T-45362_20240329_WB_M_tissue_24040123_824.webp)
![Boiled and unboiled rat tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3433] (GTX641275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641275/GTX641275_T-45586_20241108_WB_R_brain_ub_24111321_708.webp)
![IHC-P analysis of human glioblastoma (GBM) tissue using GTX641400 mGluR5 antibody [H339] HistoMAX?. Glioblastoma with moderate to strong mGluR5 staining of most tumor cells.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641400/GTX641400_20250117_IHC-P_1_25012200_610.webp)