ICC/IF analysis of COS7 cells transiently transfected with Mig-2 plasmid using GTX84506 Mig-2 antibody [14A11].
![ICC/IF analysis of HepG2 cells using GTX84506 Mig-2 antibody [14A11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84506/GTX84506_1114_ICCIF_w_23061420_614.webp "ICC/IF analysis of HepG2 cells using GTX84506 Mig-2 antibody [14A11].")
![IHC-P analysis of human pancreas tissue using GTX84506 Mig-2 antibody [14A11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84506/GTX84506_2798_IHC-P_w_23061420_210.webp "IHC-P analysis of human pancreas tissue using GTX84506 Mig-2 antibody [14A11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IP analysis of DDDDK tagged Mig-2 overexpressed HEK293T lysate using GTX84506 Mig-2 antibody [14A11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84506/GTX84506_3370_IP_w_23061420_125.webp "IP analysis of DDDDK tagged Mig-2 overexpressed HEK293T lysate using GTX84506 Mig-2 antibody [14A11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of various samples using GTX84506 Mig-2 antibody [14A11]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84506/GTX84506_3538_WB_w_23061420_101.webp "WB analysis of various samples using GTX84506 Mig-2 antibody [14A11]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of wild type and Mig-2 knock out HeLa cell lysate(10microg per lane) using GTX84506 Mig-2 antibody [14A11]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX84506/GTX84506_4427_20181228_WB_w_23061420_414.webp "WB analysis of wild type and Mig-2 knock out HeLa cell lysate(10microg per lane) using GTX84506 Mig-2 antibody [14A11]. Dilution : 1:500")
![WB analysis of HEK293T cells transfected with Mig-2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84506 Mig-2 antibody [14A11]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84506/GTX84506_4428_WB_w_23061420_497.webp "WB analysis of HEK293T cells transfected with Mig-2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84506 Mig-2 antibody [14A11]. Loading : 5 ug per lane")
![WB analysis of various cell lines using GTX84506 Mig-2 antibody [14A11]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84506/GTX84506_4429_WB_w_23061420_206.webp "WB analysis of various cell lines using GTX84506 Mig-2 antibody [14A11]. Loading : 35 ug per lane")
ICC/IF analysis of COS7 cells transiently transfected with Mig-2 plasmid using GTX84506 Mig-2 antibody [14A11].
Mig-2 antibody [14A11]
GTX84506
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetFERMT2
Overview
- SupplierGeneTex
- Product NameMig-2 antibody [14A11] - KO/KD, Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:50-1:100. IHC-P: 1:50. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID14A11
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID10979
- Target nameFERMT2
- Target descriptionFERM domain containing kindlin 2
- Target synonymsfermitin family homolog 2; fermitin family member 2; KIND2; kindlin 2; MIG2; mig-2; mitogen inducible gene 2 protein; mitogen inducible gene-2; PH domain-containing family C member 1; pleckstrin homology domain containing, family C (with FERM domain) member 1; pleckstrin homology domain containing, family C member 1; PLEKHC1; UNC112; UNC112B
- HostMouse
- IsotypeIgG2a
- Protein IDQ96AC1
- Protein NameFermitin family homolog 2
- Scientific DescriptionParticipates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.
- ReactivityCanine, Human, Monkey, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203