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ProductsBack/Mig-2 antibody [25A4]
ICC/IF analysis of HepG2 cells using GTX84505 Mig-2 antibody [25A4].
ICC/IF analysis of HepG2 cells using GTX84505 Mig-2 antibody [25A4].
ICC/IF analysis of HepG2 cells using GTX84505 Mig-2 antibody [25A4].

Mig-2 antibody [25A4]

GTX84505
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFERMT2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Mig-2 antibody [25A4] - KO/KD, Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:2000. ICC/IF: 1:100. IHC-P: 1:150. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    25A4
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10979
  • Target name
    FERMT2
  • Target description
    FERM domain containing kindlin 2
  • Target synonyms
    fermitin family homolog 2; fermitin family member 2; KIND2; kindlin 2; MIG2; mig-2; mitogen inducible gene 2 protein; mitogen inducible gene-2; PH domain-containing family C member 1; pleckstrin homology domain containing, family C (with FERM domain) member 1; pleckstrin homology domain containing, family C member 1; PLEKHC1; UNC112; UNC112B
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ96AC1
  • Protein Name
    Fermitin family homolog 2
  • Scientific Description
    Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Kindlin 2/FERMT2 using anti-Kindlin 2/FERMT2 antibody (A03653-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kindlin 2/FERMT2 antigen affinity purified polyclonal antibody (Catalog # A03653-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kindlin 2/FERMT2 at approximately 70 kDa. The expected band size for Kindlin 2/FERMT2 is at 70 kDa.
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