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ProductsBack/MIP / AQP0 / Aquaporin 0 Antibody (aa232-263)

MIP / AQP0 / Aquaporin 0 Antibody (aa232-263)

LS-C408023
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetMIP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MIP / AQP0 / Aquaporin 0 Antibody (aa232-263)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.1 - 0.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4284
  • Target name
    MIP
  • Target description
    major intrinsic protein of lens fiber
  • Target synonyms
    AQP0, CTRCT15, LIM1, MIP26, MP26, lens fiber major intrinsic protein, aquaporin 0
  • Host
    Rabbit
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Aquaporin 0 using anti-Aquaporin 0 antibody (PB9811). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat eye ball tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 0 antigen affinity purified polyclonal antibody (Catalog # PB9811) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 0 at approximately 28 kDa. The expected band size for Aquaporin 0 is at 28 kDa.
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