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MIP3 alpha antibody [11J22]

GTX53264
GeneTex
ApplicationsWestern Blot, Neutralisation/Blocking
Product group Antibodies
ReactivityMouse
TargetCcl20
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Overview

  • Supplier
    GeneTex
  • Product Name
    MIP3 alpha antibody [11J22]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    11J22
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID20297
  • Target name
    Ccl20
  • Target description
    C-C motif chemokine ligand 20
  • Target synonyms
    CKb4, LARC, MIP-3A, MIP-3[a], MIP3A, ST38, Scya20, exodus-1, C-C motif chemokine 20, CC chemokine LARC, CC chemokine ST38, MIP-3-alpha, beta-chemokine exodus-1, chemokine (C-C motif) ligand 20, liver and activation-regulated chemokine, macrophage inflammatory protein 3 alpha, small inducible cytokine subfamily A20, small-inducible cytokine A20
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDO89093
  • Protein Name
    C-C motif chemokine 20
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot using GeneTexs anti-Mouse MIP3a antibody shows detection of a band ~11 kDa in size corresponding to recombinant mouse MIP3a. Recombinant mouse MIP-3a was loaded on to an SDS-PAGE gel at 0.25 microg and after separation was transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4oC. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer) for 30 min at RT followed by reation with FemtoMax Chemiluminescent substrate. Data was collected using Bio-Rad VersaDocR 4000 MP imaging system.
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Figure 1. Western blot analysis of MIP-3 Alpha/CCL20 using anti-MIP-3 Alpha/CCL20 antibody (A00748-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIP-3 Alpha/CCL20 antigen affinity purified polyclonal antibody (Catalog # A00748-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIP-3 Alpha/CCL20 at approximately 14KD. The expected band size for MIP-3 Alpha/CCL20 is at 14KD.
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