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ProductsBack/MLKL Recombinant Antibody
Lane 1: HT29; Lane 2: HUVEC; Lane 3: Hela lysate; Probed with MLKL (3G4) Monoclonal Antibody (bsm-52256R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: HT29; Lane 2: HUVEC; Lane 3: Hela lysate; Probed with MLKL (3G4) Monoclonal Antibody (bsm-52256R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: HT29; Lane 2: HUVEC; Lane 3: Hela lysate; Probed with MLKL (3G4) Monoclonal Antibody (bsm-52256R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

MLKL Recombinant Antibody

BSM-52256R
Bioss Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetMLKL
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    MLKL (3G4) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID197259
  • Target name
    MLKL
  • Target description
    mixed lineage kinase domain like pseudokinase
  • Target synonyms
    hMLKL; mixed lineage kinase domain-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NB16
  • Protein Name
    Mixed lineage kinase domain-like protein
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MLKL using anti-MLKL antibody (A00535-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLKL antigen affinity purified polyclonal antibody (Catalog # A00535-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLKL at approximately 50 kDa. The expected band size for MLKL is at 54 kDa.
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