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Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MLKL antibody (GTX107538) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MLKL antibody (GTX107538) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MLKL antibody (GTX107538) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MLKL antibody

GTX107538
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetMLKL
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Overview

  • Supplier
    GeneTex
  • Product Name
    MLKL antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.52 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID197259
  • Target name
    MLKL
  • Target description
    mixed lineage kinase domain like pseudokinase
  • Target synonyms
    hMLKL, mixed lineage kinase domain-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NB16
  • Protein Name
    Mixed lineage kinase domain-like protein
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Zhou H, Zhou L, Guan Q, et al. Skp2-mediated MLKL degradation confers cisplatin-resistant in non-small cell lung cancer cells. Commun Biol. 2023,6(1):805. doi: 10.1038/s42003-023-05166-6
    Read this paper
  • Chen J, Wang S, Blokhuis B, et al. Cell Death Triggers Induce MLKL Cleavage in Multiple Myeloma Cells, Which may Promote Cell Death. Front Oncol. 2022,12:907036. doi: 10.3389/fonc.2022.907036
    Read this paper
  • Du J, Xiang Y, Liu H, et al. RIPK1 dephosphorylation and kinase activation by PPP1R3G/PP1γ promote apoptosis and necroptosis. Nat Commun. 2021,12(1):7067. doi: 10.1038/s41467-021-27367-5
    Read this paper
  • Zu R, Yu Z, Zhao J, et al. Quantitative analysis of phosphoproteome in necroptosis reveals a role of TRIM28 phosphorylation in promoting necroptosis-induced cytokine production. Cell Death Dis. 2021,12(11):994. doi: 10.1038/s41419-021-04290-7
    Read this paper
  • Palmer SN, Chappidi S, Pinkham C, et al. Evolutionary Profile for (Host and Viral) MLKL Indicates Its Activities as a Battlefront for Extensive Counteradaptation. Mol Biol Evol. 2021,38(12):5405-5422. doi: 10.1093/molbev/msab256
    Read this paper
  • Tonnus W, Meyer C, Steinebach C, et al. Dysfunction of the key ferroptosis-surveilling systems hypersensitizes mice to tubular necrosis during acute kidney injury. Nat Commun. 2021,12(1):4402. doi: 10.1038/s41467-021-24712-6
    Read this paper
  • Seo J, Seong D, Nam YW, et al. Beclin 1 functions as a negative modulator of MLKL oligomerisation by integrating into the necrosome complex. Cell Death Differ. 2020,27(11):3065-3081. doi: 10.1038/s41418-020-0561-9
    Read this paper
  • Dai J, Zhang C, Guo L, et al. A necroptotic-independent function of MLKL in regulating endothelial cell adhesion molecule expression. Cell Death Dis. 2020,11(4):282. doi: 10.1038/s41419-020-2483-3
    Read this paper
  • Lin YC, Chen MC, Hsieh TH, et al. CK1δ as a potential therapeutic target to treat bladder cancer. Aging (Albany NY). 2020,12(7):5764-5780. doi: 10.18632/aging.102966
    Read this paper
  • Johnston AN, Ma Y, Liu H, et al. Necroptosis-blocking compound NBC1 targets heat shock protein 70 to inhibit MLKL polymerization and necroptosis. Proc Natl Acad Sci U S A. 2020,117(12):6521-6530. doi: 10.1073/pnas.1916503117
    Read this paper

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