More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/MLKL Antibody
Western Blot analysis of K562 cells using MLKL Polyclonal Antibody
Western Blot analysis of K562 cells using MLKL Polyclonal Antibody
Western Blot analysis of K562 cells using MLKL Polyclonal Antibody

MLKL Antibody

CSB-PA003250
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMLKL
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    MLKL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID197259
  • Target name
    MLKL
  • Target description
    mixed lineage kinase domain like pseudokinase
  • Target synonyms
    hMLKL; mixed lineage kinase domain-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NB16
  • Protein Name
    Mixed lineage kinase domain-like protein
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

Related products

Product group Antibodies
RayBiotech
Anti-Mouse MLKL Antibody144-62787
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetMLKL
  • SizePrice
Product group Antibodies
References
GeneTex
MLKL antibodyGTX107538
ApplicationsImmunoPrecipitation, Western Blot
TargetMLKL
  • SizePrice
Figure 1. Western blot analysis of MLKL using anti-MLKL antibody (A00535-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLKL antigen affinity purified polyclonal antibody (Catalog # A00535-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLKL at approximately 50 kDa. The expected band size for MLKL is at 54 kDa.
Product group Antibodies
Boster Bio
Anti-MLKL Antibody Picoband(r)A00535-3-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetMLKL
  • SizePrice
Product group Antibodies
Assay Genie
Phospho-MLKL-S454 AntibodyCABP1173
TargetMLKL
  • SizePrice
Product group Antibodies
Biomatik
Mlkl Polyclonal AntibodyCAC10516
ApplicationsELISA, ImmunoHistoChemistry
TargetMLKL
  • SizePrice
Lane 1: HT29; Lane 2: HUVEC; Lane 3: Hela lysate; Probed with MLKL (3G4) Monoclonal Antibody (bsm-52256R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Product group Antibodies
Bioss Antibodies
MLKL Recombinant AntibodyBSM-52256R
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetMLKL
  • SizePrice
Product group Antibodies
MedChem Express
MLKL Antibody (YA289, Rabbit)HY-P80227
TargetMLKL
  • SizePrice
Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to plasma membrane & cell junctions.
Product group Antibodies
Atlas Antibodies
Anti-MLKL AntibodyHPA078638
ApplicationsImmunoCytoChemistry
ReactivityHuman
TargetMLKL
  • SizePrice
Product group Antibodies
Applied Biological Materials
Anti-MLKLY401187
ApplicationsWestern Blot, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MLKL Antibody (clone 8H7)LS-C768174
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetMLKL
  • SizePrice