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WB analysis of LOVO cell lysates using GTX87667 MLX antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of LOVO cell lysates using GTX87667 MLX antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of LOVO cell lysates using GTX87667 MLX antibody. The lane on the right is blocked with the synthesized peptide.

MLX antibody

GTX87667
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetMLX
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Overview

  • Supplier
    GeneTex
  • Product Name
    MLX antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6945
  • Target name
    MLX
  • Target description
    MAX dimerization protein MLX
  • Target synonyms
    MAD7, MXD7, TCFL4, TF4, bHLHd13, max-like protein X, BigMax protein, MAX-like bHLHZIP protein, MLX, MAX dimerization protein, class D basic helix-loop-helix protein 13, transcription factor-like protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UH92
  • Protein Name
    Max-like protein X
  • Scientific Description
    The product of this gene belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. This gene product may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MLX using anti-MLX antibody (A03087-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLX antigen affinity purified polyclonal antibody (Catalog # A03087-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLX at approximately 50 kDa. The expected band size for MLX is at 50 kDa.
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