Mouse anti Human CD27, conjugated with FITC

Nordic-MUbio

Mouse anti Human CD27, conjugated with FITC

7

PBMC: Add10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Flow Cytometry

Flow Cytometry

Primary antibodies

Research Use Only

Monoclonal

DEN-3

FITC

CD27

CD27 molecule

CD27 antigen; CD27L receptor; S152; S152. LPFS2; T cell activation antigen S152; T14; T-cell activation antigen CD27; TNFRSF7; Tp55; tumor necrosis factor receptor superfamily, member 7

Mouse

IgG1

CD27 antigen

CD27FITC

See expiration date on vial

Human

2°C to 8°C

12352203