Mouse anti Human CD54
0541
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetICAM1
Overview
- SupplierNordic-MUbio
- Product NameMouse anti Human CD54
- Delivery Days Customer7
- Application Supplier NotePBMC: Add10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8 C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20 C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
- ApplicationsFlow Cytometry
- Applications SupplierFlow Cytometry
- Category SupplierPrimary antibodies
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDD3.6
- ConjugateUnconjugated
- Gene ID3383
- Target nameICAM1
- Target descriptionintercellular adhesion molecule 1
- Target synonymsBB2; CD54; cell surface glycoprotein P3.58; epididymis secretory sperm binding protein; ICAM-1; intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor; P3.58
- HostMouse
- IsotypeIgG2b
- Protein IDP05362
- Protein NameIntercellular adhesion molecule 1
- Scientific DescriptionCD54
- Shelf life instructionSee expiration date on vial
- ReactivityHuman
- Reactivity SupplierHuman
- Reactivity Supplier NoteCD54=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with the major histocompatibility complex (MHC) class II-negative human b-lymphoblastoid cell line 6.16c13
- UNSPSC12352203
References
- 1. ICAM-1 a ligand for LFA-1 dependent adhesion of B,T and myeloid cells. Makgoba, M.W., Sanders, M.E., Ginther, Luce, G.E., Dustin, M.L., Springer,T.A., Clark, E.A., Mannoni, P., Shaw, S., Nature, 1988:331:86. 2. Supergene families meet in the immune system. Dustin, M.L., Staunton, D.E., and Springer, T.A., Immunol. Today. 1988:9:213. 3. Functional cloning of ICAM-2, a cell adhesion ligand for LFA-1 homologous to ICAM-1. Staunto, D.E., Dustin, M.L., and Springer, T.A., Nature. 1989:339:61. 4. Leukocytes adhesion to cells. Molecular basis, Physiological Relevance and Abnormalities. Patarroyo, M., and Makgoba, M.W., Scand. J. Immunol. 1989;30:129. 5. A soluble form of intercellular adhesion molecule-1 inhibits rhinovirus infection. Marlin SD, Staunton DE, Springer TA, Stratowa C, Sommergruber, W., and Merluzzi, V.J., Nature. 1990;44:70.