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Mouse anti Human Myogenin

X1415M
Nordic-MUbio
ApplicationsGel Shift Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMYOG
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human Myogenin
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody can be used for ELISA, gel supershift assays, immunofluorescence, immunoprecipitation of protein-DNA complexes (2 microg/mg), Western blotting (1 microg/ml) and is the antibody of choise for immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-2 microg/ml). Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Gel Shift Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA;Gel Supershift;Immunohistochemistry (paraffin);Immunofluorescence;Immunoprecipitation;Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    F12B
  • Conjugate
    Unconjugated
  • Gene ID4656
  • Target name
    MYOG
  • Target description
    myogenin
  • Target synonyms
    bHLHc3; class C basic helix-loop-helix protein 3; MYF4; myf-4; myogenic factor 4; myogenin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP15173
  • Protein Name
    Myogenin
  • Scientific Description
    Myogenin, Myogenic factor 4, Myf-4
  • Shelf life instruction
    See expiration date on vial
  • Source
    Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.
  • Reactivity Supplier
    Human;Mouse;Rat
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Myogenin/MYOG using anti-Myogenin/MYOG antibody (A03238-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey skeletal muscle tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myogenin/MYOG antigen affinity purified polyclonal antibody (Catalog # A03238-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myogenin/MYOG at approximately 40 kDa. The expected band size for Myogenin/MYOG is at 25 kDa.
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