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ProductsBack/Mouse anti Human Proximal Nephrogenic Renal Antigen, cln PN-15 (66.4.C2)

Mouse anti Human Proximal Nephrogenic Renal Antigen, cln PN-15 (66.4.C2)

X1234M
Nordic-MUbio
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCA9
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human Proximal Nephrogenic Renal Antigen, cln PN-15 (66.4.C2)
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody can be used for the differential diagnosis of carcinomas by Western blotting and immunohistochemistry on frozen and paraffin-embedded tissues. Protease digestion prior to antibody incubation enhances staining of tissue sections. Optimal concentration should be evaluated by serial dilutions.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Immunohistochemistry (paraffin);Immunohistochemistry (frozen)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    66.4.C2
  • Conjugate
    Unconjugated
  • Gene ID768
  • Target name
    CA9
  • Target description
    carbonic anhydrase 9
  • Target synonyms
    CAIX, MN, carbonic anhydrase 9, CA-IX, P54/58N, RCC-associated antigen G250, RCC-associated protein G250, carbonate dehydratase IX, carbonic anhydrase IX, carbonic dehydratase, membrane antigen MN, pMW1, renal cell carcinoma-associated antigen G250
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ16790
  • Protein Name
    Carbonic anhydrase 9
  • Shelf life instruction
    See expiration date on vial
  • Source
    Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with renal cortical tissue extract and mouse myeloma cells
  • Reactivity Supplier
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carbonic Anhydrase 9/CA9 antigen affinity purified polyclonal antibody (Catalog # A01083-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carbonic Anhydrase 9/CA9 at approximately 54 kDa. The expected band size for Carbonic Anhydrase 9/CA9 is at 50 kDa.
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