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ProductsBack/Mouse anti Lamin A and C, conjugated to FITC

Mouse anti Lamin A and C, conjugated to FITC

MUB1102L1
Nordic-MUbio
ApplicationsFlow Cytometry, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetLMNA
1 ml
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Lamin A and C, conjugated to FITC
  • Delivery Days Customer
    7
  • Application Supplier Note
    131C3 is suitable for immunocytochemistry, immunohistochemistry on frozen sections and flow cytometry. Optimal antibody dilutions for the different applications should be determined by titration. The recommended dilution is 1:10.
  • Applications
    Flow Cytometry, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    Flow Cytometry;Immunocytochemistry;Immunohistochemistry (frozen)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    131C3
  • Conjugate
    FITC
  • Gene ID4000
  • Target name
    LMNA
  • Target description
    lamin A/C
  • Target synonyms
    CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1, lamin, 70 kDa lamin, epididymis secretory sperm binding protein, lamin A/C-like 1, lamin C, mandibuloacral dysplasia type A, prelamin-A/C, progerin, renal carcinoma antigen NY-REN-32
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP02545
  • Protein Name
    Prelamin-A/C
  • Source
    131C3 is a mouse monoclonal IgG1/k antibody derived by fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with purified rat liver lamins.
  • Reactivity Supplier
    Bovine;Dog;Hamster;Human;Mouse;Rat;Sheep
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lamin A/C using anti-Lamin A/C antibody (PB9280). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin A/C antigen affinity purified polyclonal antibody (Catalog # PB9280) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin A/C at approximately 74 kDa, 63 kDa. The expected band size for Lamin A/C is at 74 kDa.
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