Mouse anti P53

Nordic-MUbio

Mouse anti P53

7

Bp53.12 reacts with a denatuRation-resistant epitope of p53. This antibody is specific for Human p53 and recognizes amino acid sequence 16-25.

Bp53.12 is useful for immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Flow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Flow Cytometry;Immunocytochemistry;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting

Primary antibodies

Research Use Only

Monoclonal

Bp53.12

TP53

tumor protein p53

antigen NY-CO-13; BCC7; BMFS5; cellular tumor antigen p53; LFS1; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; tumor supressor p53

Mouse

IgG2a

Cellular tumor antigen p53

Bp53.12 is a Mouse monoclonal IgG2a antibody derived by fusion of X63 Ag8.653 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with recombinant Human wild-type p53 protein.

Human

Human

12352203