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ProductsBack/Sheep anti Human p53

Sheep anti Human p53

A145P
Nordic-MUbio
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetTP53
250 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Sheep anti Human p53
  • Delivery Days Customer
    7
  • Application Supplier Note
    Applications: Western Blot (1-10 ug/ml), Immunoprecipitation, immunostaining of formalin-fixed and paraffine embedded tissues.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    Western Blotting;Immunoprecipitation;Immunohistochemistry
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7157
  • Target name
    TP53
  • Target description
    tumor protein p53
  • Target synonyms
    antigen NY-CO-13; BCC7; BMFS5; cellular tumor antigen p53; LFS1; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; tumor supressor p53
  • Host
    Sheep
  • Isotype
    IgG
  • Protein IDP04637
  • Protein Name
    Cellular tumor antigen p53
  • Shelf life instruction
    See expiration date on vial
  • Source
    Sheep were immunized with a GST-p53 fusion protein containing amino acids 1-393 of human p53
  • Reactivity Supplier
    Human;Mouse;Rat
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TP53 using anti-TP53 antibody (A00001-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 antigen affinity purified polyclonal antibody (Catalog # A00001-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53 at approximately 53 kDa. The expected band size for TP53 is at 44 kDa.
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IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
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