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MTDH-Specific antibody

FNAB00189
Wuhan Fine Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMTDH
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Overview

  • Supplier
    Wuhan Fine Biotech
  • Product Name
    MTDH-Specific antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Estimated Purity
    ≥95%
  • Gene ID92140
  • Target name
    MTDH
  • Target description
    metadherin
  • Target synonyms
    3D3, AEG-1, AEG1, LYRIC, LYRIC/3D3, protein LYRIC, 3D3/LYRIC, astrocyte elevated gene 1, astrocyte elevated gene-1 protein, lysine-rich CEACAM1 co-isolated protein, metastasis adhesion protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86UE4
  • Protein Name
    Protein LYRIC
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LYRIC using anti-LYRIC antibody (PB9338). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: U-87MG whole cell lysates, Lane 2: Hela whole cell lysates, Lane 3: MDA-MB-453 whole cell lysates, Lane 4: PC-3 whole cell lysates, Lane 5: A431 whole cell lysates, Lane 6: CACO-2 whole cell lysates, Lane 7: HepG2 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYRIC antigen affinity purified polyclonal antibody (Catalog # PB9338) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYRIC at approximately 70-80KD. The expected band size for LYRIC is at 70-80KD.
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