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Western Blot analysis of Human liver cancer and stomach cancer tissue using MUTYH Polyclonal Antibody at dilution of 1:1000
Western Blot analysis of Human liver cancer and stomach cancer tissue using MUTYH Polyclonal Antibody at dilution of 1:1000
Western Blot analysis of Human liver cancer and stomach cancer tissue using MUTYH Polyclonal Antibody at dilution of 1:1000

MUTYH Polyclonal Antibody

E-AB-10477
Elabscience
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMUTYH
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Overview

  • Supplier
    Elabscience
  • Product Name
    MUTYH Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4595
  • Target name
    MUTYH
  • Target description
    mutY DNA glycosylase
  • Target synonyms
    MYH, adenine DNA glycosylase, A/G-specific adenine DNA glycosylase, mutY homolog, mutY-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UIF7
  • Protein Name
    Adenine DNA glycosylase
  • Scientific Description
    This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of MUTYH using anti-MUTYH antibody (A01618-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUTYH antigen affinity purified polyclonal antibody (Catalog # A01618-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUTYH at approximately 60 kDa. The expected band size for MUTYH is at 60 kDa.
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