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ProductsBack/MX1 Mouse Monoclonal Antibody [Clone ID: OTI2G12]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MX1 (Cat# RC207418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MX1(Cat# TA500357). Positive lysates [LY400886] (100ug) and [LC400886] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MX1 (Cat# RC207418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MX1(Cat# TA500357). Positive lysates [LY400886] (100ug) and [LC400886] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MX1 (Cat# RC207418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MX1(Cat# TA500357). Positive lysates [LY400886] (100ug) and [LC400886] (20ug) can be purchased separately from OriGene.

MX1 Mouse Monoclonal Antibody [Clone ID: OTI2G12]

CF500357
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMX1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    MX1 Mouse Monoclonal Antibody [Clone ID: OTI2G12]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2G12
  • Gene ID4599
  • Target name
    MX1
  • Target description
    MX dynamin like GTPase 1
  • Target synonyms
    IFI-78K, IFI78, MX, MxA, lncMX1-215, interferon-induced GTP-binding protein Mx1, interferon-induced protein p78, interferon-inducible protein p78, interferon-regulated resistance GTP-binding protein MxA, myxoma resistance protein 1, myxovirus (influenza virus) resistance 1, interferon-inducible protein p78
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP20591
  • Protein Name
    Interferon-induced GTP-binding protein Mx1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) MX1 mouse monoclonal antibody, clone OTI2G12 (formerly 2G12)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MX1 using anti-MX1 antibody (A00849-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MX1 antigen affinity purified polyclonal antibody (Catalog # A00849-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MX1 at approximately 76 kDa. The expected band size for MX1 is at 76 kDa.
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Lane 1: T47D cell lysates probed with MX1 Monoclonal Antibody, Unconjugated (bsm-51528M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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