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MYH7 antibody [N1], N-term

GTX100713
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetMYH7
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Overview

  • Supplier
    GeneTex
  • Product Name
    MYH7 antibody [N1], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.82 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4625
  • Target name
    MYH7
  • Target description
    myosin heavy chain 7
  • Target synonyms
    cardiac muscle myosin heavy chain 7 beta; CMD1S; CMH1; MPD1; MYHCB; myHC-beta; myhc-slow; myopathy, distal 1; myosin 7; myosin heavy chain beta-subunit; myosin, heavy chain 7, cardiac muscle, beta; myosin, heavy polypeptide 7, cardiac muscle, beta; myosin-7; rhabdomyosarcoma antigen MU-RMS-40.7A; SPMD; SPMM
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12883
  • Protein Name
    Myosin-7
  • Scientific Description
    Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Effects of 17-AAG on the RIP1/RIP3/MLKL pathway during the development of heart failure following myocardial infarction in rats. Marunouchi T et al., 2021 Oct, J Pharmacol Sci
    Read more

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Figure 1. Western blot analysis of Myosin using anti-Myosin antibody (MA1064). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey heart tissue lysates, Lane 2: monkey skeletal muscle tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Myosin antigen affinity purified monoclonal antibody (Catalog # MA1064) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Myosin at approximately 230 kDa. The expected band size for Myosin is at 223 kDa.
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