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Myosin heavy chain Antibody

HY-P80234
MedChem Express
TargetMYH7
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Myosin heavy chain Antibody
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4625
  • Target name
    MYH7
  • Target description
    myosin heavy chain 7
  • Target synonyms
    cardiac muscle myosin heavy chain 7 beta; CMD1S; CMH1; MPD1; MYHCB; myHC-beta; myhc-slow; myopathy, distal 1; myosin 7; myosin heavy chain beta-subunit; myosin, heavy chain 7, cardiac muscle, beta; myosin, heavy polypeptide 7, cardiac muscle, beta; myosin-7; rhabdomyosarcoma antigen MU-RMS-40.7A; SPMD; SPMM
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Myosin heavy chain Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 224 kDa, targeting to Myosin heavy chain. It can be used for WB,IHC-P,IF assays with tag free, in the background of Human, Mouse, Rat.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Myosin using anti-Myosin antibody (MA1064). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey heart tissue lysates, Lane 2: monkey skeletal muscle tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Myosin antigen affinity purified monoclonal antibody (Catalog # MA1064) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Myosin at approximately 230 kDa. The expected band size for Myosin is at 223 kDa.
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